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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX27 Monoclonal antibody (68386-1-Ig)

    WB analysis using 68386-1-Ig (same clone as 68386-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68386-1-Ig (SNX27 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68386-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SNX27 Monoclonal antibody (68386-1-Ig)

    IF Staining of HeLa using 68386-1-Ig (same clone as 68386-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNX27 antibody (68386-1-Ig, Clone: 2C4G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68386-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SNX27 Monoclonal antibody (68386-1-Ig)

    IF Staining of HEK-293 using 68386-1-Ig (same clone as 68386-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SNX27 antibody (68386-1-Ig, Clone: 2C4G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68386-1-PBS in a different storage buffer formulation.

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SNX27 Monoclonal antibody, PBS Only

SNX27 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68386-1-PBS
Clone No.2C4G5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Sorting nexin-27, My014, KIAA0488, 2C4G5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68386-1-PBS targets SNX27 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SNX27 fusion protein Ag9447 相同性解析による交差性が予測される生物種
Full Name sorting nexin family member 27
Calculated molecular weight 61 kDa, 60 kDa, 52 kDa, 28 kDa
Observed molecular weight61 kDa
GenBank accession numberBC012184
Gene Symbol SNX27
Gene ID (NCBI) 81609
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96L92
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Sorting nexin 27 (SNX27), a PDZ domain-containing protein, belongs to the sorting nexin family of proteins. SNX27 promotes the recycling of internalized transmembrane proteins from endosomes to the plasma membrane by linking PDZ-dependent cargo recognition to retromer-mediated transport (PMID: 21602791; 23563491). It has been shown that SNX27 deficiency contributes to synaptic and cognitive deficits by modulating glutamate receptor recycling in Down's syndrome (PMID: 23524343). SNX27 has also been proposed to have a role in regulating β-amyloid (Aβ) generation by modulating γ-secretase activity, which is a molecular mechanism for Aβ-dependent pathogenesis in both Down's syndrome and Down's syndrome (PMID: 25437557).