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  • Featured Product
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SNX4 Polyclonal antibody

SNX4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11506-1-AP

Synonyms

Sorting nexin-4, sorting nexin 4

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using SNX4 Polyclonal antibody (11506-1-AP)

    WB analysis of A549 using 11506-1-AP

    WB result of SNX4 antibody (11506-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNX4 transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using SNX4 Polyclonal antibody (11506-1-AP)

    WB analysis using 11506-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11506-1-AP (SNX4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SNX4 Polyclonal antibody (11506-1-AP)

    WB analysis using 11506-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11506-1-AP (SNX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using SNX4 Polyclonal antibody (11506-1-AP)

    WB analysis of NIH/3T3 using 11506-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11506-1-AP (SNX4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using SNX4 Polyclonal antibody (11506-1-AP)

    IHC staining of human stomach using 11506-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11506-1-AP (SNX4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells, A431 cells, A549 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11506-1-AP targets SNX4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNX4 fusion protein Ag2058 相同性解析による交差性が予測される生物種
Full Name sorting nexin 4
Calculated molecular weight 450 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC018762
Gene symbol SNX4
Gene ID (NCBI) 8723
RRIDAB_2286398
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SNX4 antibody 11506-1-APDownload protocol
IHC protocol for SNX4 antibody 11506-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Recycling of autophagosomal components from autolysosomes by the recycler complex.

Authors - Chuchu Zhou
View Article