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Target

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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

SNX7 Polyclonal antibody

SNX7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12269-1-AP

Synonyms

SNX7, sorting nexin 7

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SNX7 Polyclonal antibody (12269-1-AP)

    WB analysis of HeLa using 12269-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12269-1-AP (SNX7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SNX7 Polyclonal antibody (12269-1-AP)

    IHC staining of human pancreas cancer using 12269-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12269-1-AP (SNX7 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12269-1-AP targets SNX7 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNX7 fusion protein Ag2920 相同性解析による交差性が予測される生物種
Full Name sorting nexin 7
Calculated molecular weight 45 kDa, 39 kDa, 52 kDa
Observed molecular weight 45 kDa, 52 kDa
GenBank accession numberBC018105
Gene symbol SNX7
Gene ID (NCBI) 51375
RRIDAB_10694667
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SNX7 antibody 12269-1-APDownload protocol
IHC protocol for SNX7 antibody 12269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

J Cell Sci

A heterodimeric SNX4:SNX7 SNX-BAR autophagy complex coordinates ATG9A trafficking for efficient autophagosome assembly

Authors - Zuriñe Antón
  • KD Validated
View Article
humanWB

J Cell Sci

A heterodimeric SNX4--SNX7 SNX-BAR autophagy complex coordinates ATG9A trafficking for efficient autophagosome assembly.

Authors - Zuriñe Antón
View Article