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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNX8 Polyclonal antibody

SNX8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11494-1-AP

Synonyms

Mvp1, SNX8, sorting nexin 8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SNX8 Polyclonal antibody (11494-1-AP)

    WB analysis using 11494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11494-1-AP (SNX8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SNX8 Polyclonal antibody (11494-1-AP)

    IHC staining of mouse kidney using 11494-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11494-1-AP (SNX8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using SNX8 Polyclonal antibody (11494-1-AP)

    IHC staining of mouse kidney using 11494-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11494-1-AP (SNX8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11494-1-AP targets SNX8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNX8 fusion protein Ag2055 相同性解析による交差性が予測される生物種
Full Name sorting nexin 8
Calculated molecular weight 465 aa, 53 kDa
Observed molecular weight53 kDa
GenBank accession numberBC021565
Gene symbol SNX8
Gene ID (NCBI) 29886
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sorting nexin 8 (SNX8) is a member of the sorting nexin family. Members of the sorting nexin family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX8 plays a role in the endosome-to-Golgi transport (PMID: 19782049). SNX8 can be used as a potential target for treating lysosomal storage disorder (PMID: 38519472).

Protocols

Product Specific Protocols
WB protocol for SNX8 antibody 11494-1-APDownload protocol
IHC protocol for SNX8 antibody 11494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols