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  • KD/KO Validated

SNX9 Polyclonal antibody

SNX9 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15721-1-AP

Synonyms

SH3PX1, SH3PXD3A, SH3 and PX domain-containing protein 3A, SH3 and PX domain-containing protein 1, SDP1


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Tested Applications

Positive WB detected inHeLa cells, rat heart tissue, human heart tissue, mouse skeletal muscle tissue, mouse heart tissue
Positive IP detected inmouse heart tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15721-1-AP targets SNX9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SNX9 fusion protein Ag8440 相同性解析による交差性が予測される生物種
Full Name sorting nexin 9
Calculated molecular weight 595 aa, 67 kDa
Observed molecular weight78 kDa
GenBank accession numberBC005022
Gene symbol SNX9
Gene ID (NCBI) 51429
RRIDAB_2286415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. SNX9 (Sorting nexin-9, also known as SH3PX1) was originally identified as a protein that interacted with the metalloproteinases ADAM9 and ADAM15. It contains a PX and an Sec homology 3 (SH3) domain. SNX9 functions in clathrin-mediated endocytosis and clathrin-independent, actin-dependent fluid-phase endocytosis (PMID: 17609109). On SDS-PAGE, SNX9 migrates at a molecular weight (70-78 kDa) higher than its calculated molecular mass of 67 kDa (PMID: 15299020; 12952949; 18411244; 15703209).

Protocols

Product Specific Protocols
WB protocol for SNX9 antibody 15721-1-APDownload protocol
IF protocol for SNX9 antibody 15721-1-APDownload protocol
IP protocol for SNX9 antibody 15721-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Immunity

cis-B7:CD28 interactions at invaginated synaptic membranes provide CD28 co-stimulation and promote CD8+ T cell function and anti-tumor immunity

Authors - Yunlong Zhao
mouseWB

Nat Commun

Selective packaging of mitochondrial proteins into extracellular vesicles prevents the release of mitochondrial DAMPs.

Authors - Kiran Todkar
  • KD Validated
humanIF

Cancer Discov

In vivo role of INPP4B in tumor and metastasis suppression through regulation of PI3K/AKT signaling at endosomes.

Authors - Chen Li Chew
humanWB

Dev Cell

Mitochondrial-derived vesicles compensate for loss of LC3-mediated mitophagy.

Authors - Christina G Towers
  • KD Validated
mouseWB

Proc Natl Acad Sci U S A

Loss of FCHSD1 leads to amelioration of chronic obstructive pulmonary disease.

Authors - Takahiro Kawasaki
mouseWB

Oncotarget

Sorting nexin 9 (SNX9) is not essential for development and auditory function in mice.

Authors - Chengcheng Liu
  • KO Validated