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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SOAT2 Polyclonal antibody (21852-1-AP)

    WB analysis using 21852-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21852-1-AP (SOAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using SOAT2 Polyclonal antibody (21852-1-AP)

    WB analysis of L02 using 21852-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21852-1-AP (SOAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using SOAT2 Polyclonal antibody (21852-1-AP)

    IHC staining of human liver using 21852-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21852-1-AP (SOAT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using SOAT2 Polyclonal antibody (21852-1-AP)

    IHC staining of human small intestine using 21852-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21852-1-AP (SOAT2 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using SOAT2 Polyclonal antibody (21852-1-AP)

    IHC staining of human small intestine using 21852-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21852-1-AP (SOAT2 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using SOAT2 Polyclonal antibody (21852-1-AP)

    IHC staining of human liver using 21852-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21852-1-AP (SOAT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SOAT2 Polyclonal antibody (21852-1-AP)

    IF Staining of HeLa using 21852-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SOAT2 antibody (21852-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SOAT2 Polyclonal antibody (21852-1-AP)

    IF Staining of HeLa using 21852-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SOAT2 antibody (21852-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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SOAT2 Polyclonal antibody

SOAT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21852-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:2.3.1.26, ARGP2, Acyl-coenzyme A:cholesterol acyltransferase 2, ACAT-2, ACAT2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, L02 cells
Positive IHC detected inhuman liver tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

21852-1-AP targets SOAT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SOAT2 fusion protein Ag16364 相同性解析による交差性が予測される生物種
Full Name sterol O-acyltransferase 2
Calculated molecular weight 522 aa, 60 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC096090
Gene Symbol SOAT2
Gene ID (NCBI) 8435
RRIDAB_11183043
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75908
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SOAT2, also named as ACAT2, belongs to the membrane-bound acyltransferase family and Sterol o-acyltransferase subfamily. SOAT2 is expressed preferentially in enterocytes of the intestine and hepatocytes of the liver. The role of SOAT2 in generating esterified cholesterol (EC) that is incorporated directly into atherogenic lipoproteins has made it an attractive target in dyslipidemia management and prevention. (PMID: 29246491)

Protocols

Product Specific Protocols
WB protocol for SOAT2 antibody 21852-1-APDownload protocol
IHC protocol for SOAT2 antibody 21852-1-APDownload protocol
IF protocol for SOAT2 antibody 21852-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell Death Discov

Cholesterol activates the Wnt/PCP-YAP signaling in SOAT1-targeted treatment of colon cancer.

Authors - Huanji Xu
View Article
mouseIHC

Nat Commun

Increased SOAT2 expression in aged regulatory T cells is associated with altered cholesterol metabolism and reduced anti-tumor immunity

Authors - Mingjiong Zhang
View Article