Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCIF

WB analysis using 10269-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10269-1-AP
WB result of SOD1 antibody (10269-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOD1 transfected HEK-293 cells.
WB analysis using 10269-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10269-1-AP
HeLa (WT and SOD1 KO) lysates prepared with RIPA buffer, 20 μg protein loaded. 10269-1-AP incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IP experiment of HeLa using 10269-1-AP
HeLa lysates prepared and IP of SOD1 performed using 2.0 μg of 10269-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.
IP experiment of HEK-293 using 10269-1-AP
IP result of anti-SOD1 (IP:10269-1-AP, 4ug; Detection:10269-1-AP 1:6000) with HEK-293 cells lysate 1470 ug.
IHC staining of human liver cancer using 10269-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10269-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 10269-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 10269-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 10269-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of mouse brain using 10269-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SOD1 antibody (10269-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 10269-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SOD1 antibody (10269-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 10269-1-AP
HeLa WT cells (yellow outline) and SOD1 KO cells (red outline) labelled with a green or a far-red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 10269-1-AP. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

Featured Product
KD/KO Validated

SOD1 Polyclonal antibody

SOD1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 10269-1-AP

Publications(295)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Synonyms

SOD, IPOA, hSod1, homodimer, EC:1.15.1.1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (295)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293T cells, HeLa cells, HEK-293 cells, Jurkat cells, SH-SY5Y cells, human placenta tissue, mouse brain tissue, mouse liver tissue, rat liver tissue
Positive IP detected in	HEK-293 cells, HeLa cells
Positive IHC detected in	human liver cancer tissue, human colon tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse brain tissue
Positive IF/ICC detected in	HEK-293 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 266 publications below
IHC	See 41 publications below
IF	See 16 publications below
IP	See 3 publications below
ELISA	See 1 publications below
CoIP	See 1 publications below

Product Information

10269-1-AP targets SOD1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, canine, chicken, bovine, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SOD1 fusion protein Ag0335 相同性解析による交差性が予測される生物種
Full Name	superoxide dismutase 1, soluble
Calculated molecular weight	16 kDa
Observed molecular weight	16-20 kDa
GenBank accession number	BC001034
Gene Symbol	SOD1
Gene ID (NCBI)	6647
RRID	AB_2193750
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P00441
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643).

Protocols

Product Specific Protocols
WB protocol for SOD1 antibody 10269-1-AP	Download protocol
IHC protocol for SOD1 antibody 10269-1-AP	Download protocol
IF protocol for SOD1 antibody 10269-1-AP	Download protocol
IP protocol for SOD1 antibody 10269-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cell Stem Cell Artificial extracellular matrix scaffolds of mobile molecules enhance maturation of human stem cell-derived neurons Authors - Zaida Álvarez View Article
mouse	WB	Cell Metab Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes. Authors - Calvin S Carter View Article
mouse	WB	Bioact Mater Dynamic degradation patterns of porous polycaprolactone/β-tricalcium phosphate composites orchestrate macrophage responses and immunoregulatory bone regeneration Authors - Hao Wu View Article
rat	WB	Bioact Mater A two-pronged approach to inhibit ferroptosis of MSCs caused by the iron overload in postmenopausal osteoporosis and promote osseointegration of titanium implant Authors - Yulu Yang View Article
mouse	WB	Nat Struct Mol Biol TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS. Authors - Xinxin Zuo View Article
human	WB	Nat Commun Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis. Authors - Yun Liao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10269-1-AP

WB analysis of HEK-293 using 10269-1-AP

WB analysis using 10269-1-AP

WB analysis of HeLa using 10269-1-AP

IP experiment of HeLa using 10269-1-AP

IP experiment of HEK-293 using 10269-1-AP

IHC staining of human liver cancer using 10269-1-AP

IHC staining of human liver cancer using 10269-1-AP

IHC staining of human colon using 10269-1-AP

IHC staining of human heart using 10269-1-AP

IHC staining of human heart using 10269-1-AP

IF Staining of mouse brain using 10269-1-AP

IF Staining of HEK-293 using 10269-1-AP

IF Staining of HeLa using 10269-1-AP

SOD1 Polyclonal antibody

SOD1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Artificial extracellular matrix scaffolds of mobile molecules enhance maturation of human stem cell-derived neurons

Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.

Dynamic degradation patterns of porous polycaprolactone/β-tricalcium phosphate composites orchestrate macrophage responses and immunoregulatory bone regeneration

A two-pronged approach to inhibit ferroptosis of MSCs caused by the iron overload in postmenopausal osteoporosis and promote osseointegration of titanium implant

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis.