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  • Featured Product
  • KD/KO Validated

SOD1 Polyclonal antibody

SOD1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10269-1-AP

Synonyms

SOD, IPOA, hSod1, homodimer, EC:1.15.1.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SOD1 Polyclonal antibody (10269-1-AP)

    WB analysis using 10269-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SOD1 Polyclonal antibody (10269-1-AP)

    WB analysis of HEK-293 using 10269-1-AP

    WB result of SOD1 antibody (10269-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SOD1 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using SOD1 Polyclonal antibody (10269-1-AP)

    WB analysis using 10269-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10269-1-AP (SOD1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SOD1 Polyclonal antibody (10269-1-AP)

    WB analysis of HeLa using 10269-1-AP

    HeLa (WT and SOD1 KO) lysates prepared with RIPA buffer, 20 μg protein loaded. 10269-1-AP incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • Immunoprecipitation (IP) experiment of HeLa cells using SOD1 Polyclonal antibody (10269-1-AP)

    IP experiment of HeLa using 10269-1-AP

    HeLa lysates prepared and IP of SOD1 performed using 2.0 μg of 10269-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using SOD1 Polyclonal antibody (10269-1-AP)

    IP experiment of HEK-293 using 10269-1-AP

    IP result of anti-SOD1 (IP:10269-1-AP, 4ug; Detection:10269-1-AP 1:6000) with HEK-293 cells lysate 1470 ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SOD1 Polyclonal antibody (10269-1-AP)

    IHC staining of human liver cancer using 10269-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SOD1 Polyclonal antibody (10269-1-AP)

    IHC staining of human liver cancer using 10269-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using SOD1 Polyclonal antibody (10269-1-AP)

    IHC staining of human colon using 10269-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using SOD1 Polyclonal antibody (10269-1-AP)

    IHC staining of human heart using 10269-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using SOD1 Polyclonal antibody (10269-1-AP)

    IHC staining of human heart using 10269-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10269-1-AP (SOD1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SOD1 Polyclonal antibody (10269-1-AP)

    IF Staining of mouse brain using 10269-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SOD1 antibody (10269-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SOD1 Polyclonal antibody (10269-1-AP)

    IF Staining of HEK-293 using 10269-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SOD1 antibody (10269-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SOD1 Polyclonal antibody (10269-1-AP)

    IF Staining of HeLa using 10269-1-AP

    HeLa WT cells (yellow outline) and SOD1 KO cells (red outline) labelled with a green or a far-red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 10269-1-AP. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HEK-293 cells, Jurkat cells, SH-SY5Y cells, human placenta tissue, mouse brain tissue, mouse liver tissue, rat liver tissue
Positive IP detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human colon tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10269-1-AP targets SOD1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine, chicken, bovine, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SOD1 fusion protein Ag0335 相同性解析による交差性が予測される生物種
Full Name superoxide dismutase 1, soluble
Calculated molecular weight 16 kDa
Observed molecular weight 16-20 kDa
GenBank accession numberBC001034
Gene symbol SOD1
Gene ID (NCBI) 6647
RRIDAB_2193750
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The enzymatic function of Cu/Zn Superoxide Dismutase (SOD1), previously known as hemocuprein and IPOA, was first characterized in 1969 (PMID: 5389100). SOD1 is commonly known for its ROS scavenging activity, but recent work has uncovered additional roles in modulating metabolism, maintaining redox balance, and regulating transcription. In disease contexts, SOD1 is best-known for its role in a familial form of amyotrophic lateral sclerosis (fALS) (PMID: 10630188). In addition, SOD1 is overexpressed in numerous cancer types, including lung adenocarcinoma, non-small-cell lung cancer , and 70% of primary breast cancers (PMID: 31344643).

Protocols

Product Specific Protocols
WB protocol for SOD1 antibody 10269-1-APDownload protocol
IHC protocol for SOD1 antibody 10269-1-APDownload protocol
IF protocol for SOD1 antibody 10269-1-APDownload protocol
IP protocol for SOD1 antibody 10269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Stem Cell

Artificial extracellular matrix scaffolds of mobile molecules enhance maturation of human stem cell-derived neurons

Authors - Zaida Álvarez
View Article
mouseWB

Cell Metab

Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.

Authors - Calvin S Carter
View Article
mouseWB

Bioact Mater

Dynamic degradation patterns of porous polycaprolactone/β-tricalcium phosphate composites orchestrate macrophage responses and immunoregulatory bone regeneration

Authors - Hao Wu
View Article
mouseWB

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
View Article
humanWB

Nat Commun

Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis.

Authors - Yun Liao
View Article
pigWB

Microbiome

Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets.

Authors - Leli Wang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Scott (Verified Customer) (10-22-2024)

20µg of protein was loaded and antibody was incubated overnight at 4oC following a total protein stain. The band appeared at the expected size. Precision plus protein standard ladder #1610373. First 5 lanes are Transgenic SOD1G93A mice, last four lanes are non-transgenic mice.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: SOD1G93A Mouse Brain Lysate
SOD1 Antibody Western Blot validation (1:5000 dilution) in SOD1G93A Mouse Brain Lysate (Cat no:10269-1-AP)
FH

Tianyi (Verified Customer) (08-17-2023)

Paraffin-embedded tissues (4% PFA, 24 h) incubated with SOD1 (1:200) and secondary antibody Alexa Fluor donkey anti-rabbit 594 IgG (H+L).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Ovarian tissue
SOD1 Antibody Immunofluorescence validation (1:200 dilution) in Ovarian tissue (Cat no:10269-1-AP)
FH

Xingyuan (Verified Customer) (07-17-2023)

Quality was decent.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:15000
  • Cell Tissue Type: 293T
SOD1 Antibody Western Blot validation (1:15000 dilution) in 293T (Cat no:10269-1-AP)
FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
FH

Elise (Verified Customer) (06-24-2022)

Tested on Western blot for 20µg of protein from kidney tissue. Antibody diluted at 1 :1000 in TBS-T BSA 5 %

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Kidney tissue
FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by SOD1 Polyclonal antibody at dilution of 1:100 showed strong labelling.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: NSC-34 cells