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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SOD1 Polyclonal antibody (31400-1-AP)

    WB analysis using 31400-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31400-1-AP (SOD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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SOD1 Polyclonal antibody

SOD1 Polyclonal Antibody for WB, ELISA
Cat No. 31400-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, mouse liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31400-1-AP targets SOD1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name superoxide dismutase 1, soluble
Calculated molecular weight16kDa
Observed molecular weight 16-20 kDa
GenBank accession numberNM_000454.5
Gene Symbol SOD1
Gene ID (NCBI) 6647
RRIDAB_3669965
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP00441
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) (SOD1, synonyms: ALS, SOD, ALS1, IPOA) binds copper and zinc ions and is one of two isozymes responsible for destroying free uperoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis.

Protocols

Product Specific Protocols
WB protocol for SOD1 antibody 31400-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols