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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SOD2 Recombinant antibody

SOD2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240462F8

Cat no : 83519-2-RR

Synonyms

SODM, MNSOD, Mn SOD, IPOB, IPO B

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SOD2 Recombinant antibody (83519-2-RR)

    IF Staining of HepG2 using 83519-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SOD2 antibody (83519-2-RR, Clone: 240462F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SOD2 Recombinant antibody (83519-2-RR)

    IF Staining of HeLa using 83519-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SOD2 antibody (83519-2-RR, Clone: 240462F8 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using SOD2 Recombinant antibody (83519-2-RR)

    FC experiment of HeLa using 83519-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SOD2 Recombinant antibody (83519-2-RR, Clone:240462F8) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83519-2-RR

    Affinity and Kinetic Characterization of 83519-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83519-2-RR against Human SOD2 were performed. The affinity constant is 2.75 nM.

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Tested Applications

Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83519-2-RR is SOD2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SOD2 fusion protein Ag21388 相同性解析による交差性が予測される生物種
Full Name superoxide dismutase 2, mitochondrial
Calculated molecular weight 25 kDa
GenBank accession numberBC016934
Gene symbol SOD2
Gene ID (NCBI) 6648
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa. This antibody is specific to SOD2.

Protocols

Product Specific Protocols
IF protocol for SOD2 antibody 83519-2-RRDownload protocol
FC protocol for SOD2 antibody 83519-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols