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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SOD2 Recombinant antibody

SOD2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240462H8

Cat no : 83519-4-RR

Synonyms

SODM, MNSOD, Mn SOD, IPOB, IPO B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SOD2 Recombinant antibody (83519-4-RR)

    WB analysis using 83519-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83519-4-RR (SOD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SOD2 Recombinant antibody (83519-4-RR)

    IHC staining of human ovary cancer using 83519-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83519-4-RR (SOD2 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SOD2 Recombinant antibody (83519-4-RR)

    IHC staining of human ovary cancer using 83519-4-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83519-4-RR (SOD2 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using SOD2 Recombinant antibody (83519-4-RR)

    IF Staining of HUVEC using 83519-4-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using SOD2 antibody (83519-4-RR, Clone: 240462H8 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Flow cytometry (FC) experiment of HL-60 cells using SOD2 Recombinant antibody (83519-4-RR)

    FC experiment of HL-60 using 83519-4-RR

    1x10^6 HL-60 cells were intracellularly stained with 0.25 ug Sod2 Recombinant Antibody (83519-4-RR, Clone:240462H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of A549 cells using SOD2 Recombinant antibody (83519-4-RR)

    FC experiment of A549 using 83519-4-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Sod2 Recombinant Antibody (83519-4-RR, Clone:240462H8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83519-4-RR

    Affinity and Kinetic Characterization of 83519-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83519-4-RR against Human SOD2 were performed. The affinity constant is 219 nM.

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HEK-293 cells, SH-SY5Y cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells
Positive FC (Intra) detected inA549 cells, HL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83519-4-RR is SOD2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SOD2 fusion protein Ag21388 相同性解析による交差性が予測される生物種
Full Name superoxide dismutase 2, mitochondrial
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC016934
Gene symbol SOD2
Gene ID (NCBI) 6648
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.

Protocols

Product Specific Protocols
WB protocol for SOD2 antibody 83519-4-RRDownload protocol
IHC protocol for SOD2 antibody 83519-4-RRDownload protocol
IF protocol for SOD2 antibody 83519-4-RRDownload protocol
FC protocol for SOD2 antibody 83519-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols