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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse colon tissue using SON Polyclonal antibody (28046-1-AP)

    IHC staining of mouse colon using 28046-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28046-1-AP (SON antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using SON Polyclonal antibody (28046-1-AP)

    IHC staining of human thyroid cancer using 28046-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 28046-1-AP (SON antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using SON Polyclonal antibody (28046-1-AP)

    IHC staining of human thyroid cancer using 28046-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 28046-1-AP (SON antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SON Polyclonal antibody

SON Polyclonal Antibody for IHC, ELISA
Cat No. 28046-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

SON3, SON DNA binding protein, KIAA1019, HSPC312, DBP5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman thyroid cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28046-1-AP targets SON in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SON fusion protein Ag27620 相同性解析による交差性が予測される生物種
Full Name SON DNA binding protein
Calculated molecular weight 264 aa
GenBank accession numberNM_001291411
Gene Symbol SON
Gene ID (NCBI) 6651
RRIDAB_3086024
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18583
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SON, also called C21orf50 and DBP5, is a nuclear speckle-localized protein that shares homology with pre-mRNA splicing accessory factors (PMID: 10950926). It is an RNA-binding protein that acts as an mRNA splicing cofactor by promoting efficient splicing of transcripts that possess weak splice sites, and specifically promotes splicing of many cell-cycle and DNA-repair transcripts that possess weak splice sites, such as TUBG1, KATNB1, TUBGCP2, AURKB, PCNT, AKT1, RAD23A, and FANCG (PMID: 20581448; 21504830).

Protocols

Product Specific Protocols
IHC protocol for SON antibody 28046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols