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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SON Recombinant antibody (83787-5-RR)

    WB analysis using 83787-5-RR (same clone as 83787-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83787-5-RR (SON antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83787-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using SON Recombinant antibody (83787-5-RR)

    IHC staining of human colon using 83787-5-RR (same clone as 83787-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 83787-5-RR (SON antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83787-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SON Recombinant antibody (83787-5-RR)

    IF Staining of U2OS using 83787-5-RR (same clone as 83787-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SON antibody (83787-5-RR, Clone: 240845B7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83787-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SON Recombinant antibody (83787-5-RR)

    IF Staining of U2OS using 83787-5-RR (same clone as 83787-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SON antibody (83787-5-RR, Clone: 240845B7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83787-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using SON Recombinant antibody (83787-5-RR)

    FC experiment of A431 using 83787-5-RR (same clone as 83787-5-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug SON Recombinant antibody (83787-5-RR, Clone:240845B7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83787-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using SON Recombinant antibody (83787-5-RR)

    FC experiment of HeLa using 83787-5-RR (same clone as 83787-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SON Recombinant antibody (83787-5-RR, Clone:240845B7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83787-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83787-5-RR

    Affinity and Kinetic Characterization of 83787-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83787-5-RR against Human SON were performed. The affinity constant is 0.591 nM.

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SON Recombinant antibody, PBS Only

SON Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83787-5-PBS
Clone No.240845B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

SON DNA binding protein, KIAA1019, HSPC312, DBP5, C21orf50

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83787-5-PBS targets SON in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SON fusion protein Ag27620 相同性解析による交差性が予測される生物種
Full Name SON DNA binding protein
Calculated molecular weight 264 aa
Observed molecular weight 250-300 kDa
GenBank accession numberNM_001291411
Gene Symbol SON
Gene ID (NCBI) 6651
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP18583
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SON, also called C21orf50 and DBP5, is a nuclear speckle-localized protein that shares homology with pre-mRNA splicing accessory factors (PMID: 10950926). It is an RNA-binding protein that acts as an mRNA splicing cofactor by promoting efficient splicing of transcripts that possess weak splice sites, and specifically promotes splicing of many cell-cycle and DNA-repair transcripts that possess weak splice sites, such as TUBG1, KATNB1, TUBGCP2, AURKB, PCNT, AKT1, RAD23A, and FANCG (PMID: 20581448; 21504830).