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Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using SORCS1 Polyclonal antibody (18839-1-AP)

    WB analysis of Y79 using 18839-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 18839-1-AP (SORCS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SORCS1 Polyclonal antibody (18839-1-AP)

    WB analysis of mouse brain using 18839-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18839-1-AP (SORCS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SORCS1 Polyclonal antibody (18839-1-AP)

    WB analysis of HEK-293 using 18839-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18839-1-AP (SORCS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using SORCS1 Polyclonal antibody (18839-1-AP)

    WB analysis of Y79 using 18839-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 18839-1-AP (SORCS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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SORCS1 Polyclonal antibody

SORCS1 Polyclonal Antibody for WB, ELISA
Cat No. 18839-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

hSorCS, SORCS1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inY79 cells, HEK-293 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18839-1-AP targets SORCS1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name sortilin-related VPS10 domain containing receptor 1
Calculated molecular weight 1168 aa, 130 kDa
Observed molecular weight 130 kDa, 60-80 kDa
GenBank accession numberBC131597
Gene Symbol SORCS1
Gene ID (NCBI) 114815
RRIDAB_2878564
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WY21
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SorCS1 is a 130 kDa type I transmembrane protein, belonging to Vps10p-domain (Vps10p-D) receptor family that comprises an N-terminal Vps10p-D, a leucine-rich domain, a single transmembrane domain, and a short cytoplasmic domain. SorCS1 is synthesized as a proprotein, which is converted to the mature receptor in late Golgi compartments by cleavage of the N-terminal propeptide. Alternative splicing of human SorCS1 results in four isoforms with different cytoplasmic domains and differential expression in tissues. This antibody was generated against the C-terminal region of human SorCS1 and recognizes various forms of SorCS1. Multiple bands around 100-130 kDa as well as 60-80 kDa have been detected in Y79 cells by western blot assay.

Protocols

Product Specific Protocols
WB protocol for SORCS1 antibody 18839-1-APDownload protocol
FC protocol for SORCS1 antibody 18839-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols