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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SORCS1 Polyclonal antibody

SORCS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23002-1-AP

Synonyms

VPS10 domain-containing receptor SorCS1, SORCS, hSorCS

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SORCS1 Polyclonal antibody (23002-1-AP)

    WB analysis using 23002-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23002-1-AP (SORCS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SORCS1 Polyclonal antibody (23002-1-AP)

    WB analysis of mouse kidney using 23002-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23002-1-AP (SORCS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using SORCS1 Polyclonal antibody (23002-1-AP)

    WB analysis of human brain using 23002-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 23002-1-AP (SORCS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using SORCS1 Polyclonal antibody (23002-1-AP)

    IHC staining of human brain using 23002-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23002-1-AP (SORCS1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using SORCS1 Polyclonal antibody (23002-1-AP)

    IHC staining of human brain using 23002-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23002-1-AP (SORCS1 Antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of HeLa cells using SORCS1 Polyclonal antibody (23002-1-AP)

    FC experiment of HeLa using 23002-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SORCS1 Polyclonal antibody (23002-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue, human brain tissue, Y79 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

23002-1-AP targets SORCS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SORCS1 fusion protein Ag19199 相同性解析による交差性が予測される生物種
Full Name sortilin-related VPS10 domain containing receptor 1
Calculated molecular weight 1168 aa, 130 kDa
Observed molecular weight 60-80 kDa
GenBank accession numberBC131597
Gene symbol SORCS1
Gene ID (NCBI) 114815
RRIDAB_2879196
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SORCS1, also named as SorCS, belongs to the family of VPS10 domain-containing receptors. SorCS1 immunoreactivity is widespread in a population of neurons throughout the brain. Two different types of cellular localization were observed. Most SorCS1 immunoreactive neurons exhibit a punctate cytoplasmic staining which extends into the dendrites, whilst occassionally SorCS1 neuronal immunoreactivity is associated with the plasma membrane.

Protocols

Product Specific Protocols
WB protocol for SORCS1 antibody 23002-1-APDownload protocol
IHC protocol for SORCS1 antibody 23002-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

CircSPIDR acts as a tumour suppressor in cervical adenocarcinoma by sponging miR-431-5p and regulating SORCS1 and CUBN expression.

Authors - Junfen Xu
View Article