Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, Y79 cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
23002-1-AP targets SORCS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SORCS1 fusion protein Ag19199 相同性解析による交差性が予測される生物種 |
| Full Name | sortilin-related VPS10 domain containing receptor 1 |
| Calculated molecular weight | 1168 aa, 130 kDa |
| GenBank accession number | BC131597 |
| Gene Symbol | SORCS1 |
| Gene ID (NCBI) | 114815 |
| RRID | AB_2879196 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q8WY21 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SORCS1, also named as SorCS, belongs to the family of VPS10 domain-containing receptors. SorCS1 immunoreactivity is widespread in a population of neurons throughout the brain. Two different types of cellular localization were observed. Most SorCS1 immunoreactive neurons exhibit a punctate cytoplasmic staining which extends into the dendrites, whilst occassionally SorCS1 neuronal immunoreactivity is associated with the plasma membrane.The predicted molecular weight (MW) of SORCS1 is 130 kDa, and the 60-80 kDa bands observed may represent degradation products.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SORCS1 antibody 23002-1-AP | Download protocol |
| IHC protocol for SORCS1 antibody 23002-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) CircSPIDR acts as a tumour suppressor in cervical adenocarcinoma by sponging miR-431-5p and regulating SORCS1 and CUBN expression. |