SOS1 Polyclonal antibody

Name: SOS1 Polyclonal antibody
Brand: Proteintech
SKU: 55041-1-AP

SOS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 55041-1-AP

Synonyms

GF1, GGF1, GINGF, NS4, Son of sevenless homolog 1, SOS 1, SOS1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 55041-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55041-1-AP (SOS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 55041-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55041-1-AP (SOS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 55041-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 55041-1-AP (SOS1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 55041-1-AP
IP result of anti-SOS1 (IP:55041-1-AP, 4ug; Detection:55041-1-AP 1:500) with mouse brain tissue lysate 4000ug.
IHC staining of mouse colon using 55041-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55041-1-AP (SOS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 55041-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55041-1-AP (SOS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 55041-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55041-1-AP (SOS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 55041-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SOS1 antibody (55041-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 55041-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	K-562 cells, mouse brain tissue, THP-1 cells, SKOV-3 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse colon tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below

Product Information

55041-1-AP targets SOS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	son of sevenless homolog 1 (Drosophila)
Calculated molecular weight	152 kDa
Observed molecular weight	152 kDa
GenBank accession number	NM_005633
Gene symbol	SOS1
Gene ID (NCBI)	6654
RRID	AB_10804659
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SOS1 promotes the exchange of Ras-bound GDP by GTP. EPS8, E3B1 and SOS1 form a tricomplex in vivo that exhibits RAC-specific guanine nucleotide exchange factor activity in vitro. Defects in SOS1 are the cause of gingival fibromatosis 1 (GGF1). Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4). The antibody is specific to SOS1.

Protocols

Product Specific Protocols
WB protocol for SOS1 antibody 55041-1-AP	Download protocol
IHC protocol for SOS1 antibody 55041-1-AP	Download protocol
IF protocol for SOS1 antibody 55041-1-AP	Download protocol
IP protocol for SOS1 antibody 55041-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cells Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer. Authors - Damian Kołat View Article
human	WB	Pathol Res Pract KBTBD2 promotes proliferation and migration of gastric cancer via activating EGFR signaling pathway Authors - Jishuang Ding View Article
human	WB	Cancers (Basel) Lactate Oxidase Disrupts Lactate-Activated RAS and PI3K Oncogenic Signaling Authors - Chandler R Keller View Article
human	WB	Sci Adv HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis Authors - Qiwei Jiang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SOS1 Polyclonal antibody

SOS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 55041-1-AP

WB analysis using 55041-1-AP

WB analysis of mouse brain using 55041-1-AP

IP experiment of mouse brain using 55041-1-AP

IHC staining of mouse colon using 55041-1-AP

IHC staining of mouse colon using 55041-1-AP

IHC staining of human lung cancer using 55041-1-AP

IF Staining of HeLa using 55041-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer.

KBTBD2 promotes proliferation and migration of gastric cancer via activating EGFR signaling pathway

Lactate Oxidase Disrupts Lactate-Activated RAS and PI3K Oncogenic Signaling

HPIP is an essential scaffolding protein running through the EGFR-RAS-ERK pathway and drives tumorigenesis