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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SOSTDC1 Polyclonal antibody (22213-1-AP)

    WB analysis using 22213-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22213-1-AP (SOSTDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Saos-2 cells using SOSTDC1 Polyclonal antibody (22213-1-AP)

    IF Staining of Saos-2 using 22213-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using SOSTDC1 antibody (22213-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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SOSTDC1 Polyclonal antibody

SOSTDC1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 22213-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

CDA019, Ectodermal BMP inhibitor, ECTODIN, sclerostin domain containing 1, SOSTDC1, USAG 1, USAG1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman milk
Positive IF/ICC detected inSaos-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22213-1-AP targets SOSTDC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SOSTDC1 fusion protein Ag17538 相同性解析による交差性が予測される生物種
Full Name sclerostin domain containing 1
Calculated molecular weight 206 aa, 23 kDa
Observed molecular weight 23-26 kDa
GenBank accession numberBC008484
Gene Symbol SOSTDC1
Gene ID (NCBI) 25928
RRIDAB_3085688
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6X4U4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SOSTDC1 (Sclerostin domain-containing protein 1), also known as Ectodin (Ectodermal BMP inhibitor), is a member of the sclerostin family. It is an N-glycosylated, secreted protein with a C-terminal cystine knot-like domain and is highly abundant in milk and kidney. This protein functions as a bone morphogenetic protein (BMP) antagonist. Specifically, it directly associates with BMPs, prohibiting them from binding their receptors, thereby regulating BMP signaling during cellular proliferation, differentiation, and programmed cell death.

Protocols

Product Specific Protocols
WB protocol for SOSTDC1 antibody 22213-1-APDownload protocol
IF protocol for SOSTDC1 antibody 22213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols