SOX10 Polyclonal antibody

SOX10 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10422-1-AP

Synonyms

WS2E, Transcription factor SOX-10, Transcription factor SOX 10, SRY-box transcription factor 9, PCWH


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Tested Applications

Positive WB detected inmouse brain tissue, mouse small intestine tissue, SH-SY5Y cells
Positive IP detected inmouse colon tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10422-1-AP targets SOX10 in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SOX10 fusion protein Ag0673 相同性解析による交差性が予測される生物種
Full Name SRY (sex determining region Y)-box 10
Calculated molecular weight 50 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC002824
Gene symbol SOX10
Gene ID (NCBI) 6663
RRIDAB_2195371
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sox10 is a transcription factor that has a crucial role in complex signalling pathways regulating central nervous system and neural crest development. The calcualted molecular weight of SOX10 is 50 kDa, but modified SOX10 is about 55-60 kDa. (PMID: 23799842)

Protocols

Product Specific Protocols
WB protocol for SOX10 antibody 10422-1-APDownload protocol
IHC protocol for SOX10 antibody 10422-1-APDownload protocol
IP protocol for SOX10 antibody 10422-1-APDownload protocol
FC protocol for SOX10 antibody 10422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nature

Regulation of intestinal immunity and tissue repair by enteric glia

Authors - Fränze Progatzky
mouseIF

Neuron

Gut-induced alpha-Synuclein and Tau propagation initiate Parkinson's and Alzheimer's disease co-pathology and behavior impairments

Authors - Jie Xiang
mouse,ratWB,IF,CoIP

Dev Cell

Nuclear import carrier Hikeshi cooperates with HSP70 to promote murine oligodendrocyte differentiation and CNS myelination

Authors - Li Li
humanIF

Front Immunol

Machine learning-based identification of SOX10 as an immune regulator of macrophage in gliomas

Authors - Gelei Xiao
mouseWB

J Cereb Blood Flow Metab

The role of serum/glucocorticoid-regulated kinase 1 in brain function following cerebral ischemia

Authors - Celeste Yin-Chieh Wu
ratIF

Sci Rep

Schwann cell-encapsulated chitosan-collagen hydrogel nerve conduit promotes peripheral nerve regeneration in rodent sciatic nerve defect models

Authors - Hiroaki Takeya