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  • Featured Product
  • KD/KO Validated

SOX2 Monoclonal antibody, PBS Only

SOX2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4B9B10

Cat no : 66411-1-PBS

Synonyms

Transcription factor SOX 2, SOX 2, MCOPS3, MCOPS 3, ANOP3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SOX2 Monoclonal antibody (66411-1-Ig)

    WB analysis using 66411-1-Ig (same clone as 66411-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SOX2 Monoclonal antibody (66411-1-Ig)

    WB analysis of HEK-293 using 66411-1-Ig (same clone as 66411-1-PBS)

    WB result of SOX2 antibody (66411-1-Ig, 1:4000) with si-Control and si-SOX2 transfected HEK293 cells.. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-251 cells using SOX2 Monoclonal antibody (66411-1-Ig)

    WB analysis of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

    WB result of SOX2 antibody (66411-1-Ig; 1:3000; room temperature for 1.5 hours) with negative control and SOX2 knockout U-251 cells. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-251 cells using SOX2 Monoclonal antibody (66411-1-Ig)

    WB analysis of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    WB analysis of fetal human brain using 66411-1-Ig (same clone as 66411-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    WB analysis of pig brain using 66411-1-Ig (same clone as 66411-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using SOX2 Monoclonal antibody (66411-1-Ig)

    WB analysis of MCF-7 using 66411-1-Ig (same clone as 66411-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    WB analysis of pig brain using 66411-1-Ig (same clone as 66411-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IHC staining of human lung cancer using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IHC staining of mouse embryo using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IHC staining of human lung cancer using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IHC staining of mouse embryo using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IHC staining of mouse embryo using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IHC staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66411-1-Ig (SOX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IHC staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66411-1-Ig (SOX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using SOX2 Monoclonal antibody (66411-1-Ig)

    IF Staining of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-251 cells using SOX2 Monoclonal antibody (66411-1-Ig)

    FC experiment of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

    1X10^6 U-251 cells were intracellularly stained with 0.5 ug Anti-Human SOX2 (66411-1-Ig, Clone:4B9B10) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66411-1-PBS targets SOX2 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityrat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SOX2 fusion protein Ag13635 相同性解析による交差性が予測される生物種
Full Name SRY (sex determining region Y)-box 2
Calculated molecular weight 34 kDa
Observed molecular weight 34-40 kDa
GenBank accession numberBC013923
Gene symbol SOX2
Gene ID (NCBI) 6657
RRIDAB_2881783
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Sox2, also known as SRY (sex determining region Y)-box 2, is a transcription factor essential for maintaining self-renewal of undifferentiated ES cells and is one of the key transcription factors used to reprogram mouse and human fibroblasts to a pluripotent state. Sox2 expressed in undifferentiated pluripotent stem cells and germ cells during development. This antibody is raised against an internal region of human SOX2.