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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SOX2 Monoclonal antibody (66411-1-Ig)

    WB analysis using 66411-1-Ig (same clone as 66411-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SOX2 Monoclonal antibody (66411-1-Ig)

    WB analysis of HEK-293 using 66411-1-Ig (same clone as 66411-1-PBS)

    WB result of SOX2 antibody (66411-1-Ig, 1:4000) with si-Control and si-SOX2 transfected HEK293 cells.. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-251 cells using SOX2 Monoclonal antibody (66411-1-Ig)

    WB analysis of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

    WB result of SOX2 antibody (66411-1-Ig; 1:3000; room temperature for 1.5 hours) with negative control and SOX2 knockout U-251 cells. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-251 cells using SOX2 Monoclonal antibody (66411-1-Ig)

    WB analysis of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    WB analysis of fetal human brain using 66411-1-Ig (same clone as 66411-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    WB analysis of pig brain using 66411-1-Ig (same clone as 66411-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using SOX2 Monoclonal antibody (66411-1-Ig)

    WB analysis of MCF-7 using 66411-1-Ig (same clone as 66411-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    WB analysis of pig brain using 66411-1-Ig (same clone as 66411-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66411-1-Ig (SOX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IHC staining of human lung cancer using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IHC staining of mouse embryo using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IHC staining of human lung cancer using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66411-1-Ig (SOX2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IHC staining of human lung cancer using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IHC staining of mouse embryo using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IHC staining of mouse embryo using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 66411-1-Ig (SOX2 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IHC staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66411-1-Ig (SOX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IHC staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66411-1-Ig (SOX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SOX2 Monoclonal antibody (66411-1-Ig)

    IF Staining of mouse brain using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using SOX2 Monoclonal antibody (66411-1-Ig)

    IF Staining of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SOX2 antibody (66411-1-Ig, Clone: 4B9B10 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-251 cells using SOX2 Monoclonal antibody (66411-1-Ig)

    FC experiment of U-251 using 66411-1-Ig (same clone as 66411-1-PBS)

    1X10^6 U-251 cells were intracellularly stained with 0.5 ug Anti-Human SOX2 (66411-1-Ig, Clone:4B9B10) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66411-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SOX2 Monoclonal antibody, PBS Only

SOX2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 66411-1-PBS
Clone No.4B9B10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Synonyms

Transcription factor SOX 2, SOX 2, MCOPS3, MCOPS 3, ANOP3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66411-1-PBS targets SOX2 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SOX2 fusion protein Ag13635 相同性解析による交差性が予測される生物種
Full Name SRY (sex determining region Y)-box 2
Calculated molecular weight 34 kDa
Observed molecular weight 34-40 kDa
GenBank accession numberBC013923
Gene Symbol SOX2
Gene ID (NCBI) 6657
RRIDAB_2881783
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP48431
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Background

SRY (sex determining region Y)-box 2 is a transcription factor involved in neural stem cell maintenance and has been used in somatic cell reprogramming.

 What is the molecular weight of Sox2?

34 kDa. Sox2 is an intronless gene encoding xx amino acids including HMG (High-mobility group) box domains that can bind to DNA.

 What is the tissue specificity of Sox2?

In embryonic stem cells, Sox2 is highly expressed and is essential in self-renewal. In the developing embryo, Sox2 is found in the neural tube and then in the developing eye and central nervous system (CNS). This expression is highly conserved across organisms (PMID: 25126380; PMID: 8625802).

In adult tissues Sox2 is expressed in neural progenitor cells, which as multipotent cells can both self-renew and differentiate into neurons, astroglia, and oligodendroglia. It maintains their stem cell phenotype and is a useful marker of early neural stem cells (PMID: 16139372).

 What is the role of Sox2 in pluripotency?

Sox2 acts as part of the LIF (Leukemia inhibitory factor) pathway, activated via JAK-STAT signaling to form complexes with other transcription factors such as Oct4 that bind to DNA and work to control pluripotency. Sox2 was described as one of the four original factors that are key to the reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) (PMID: 16904174).

 What is the role of Sox2 in cancer?

The ability to control pluripotency means dysregulation of Sox2 can lead to uncontrolled proliferation. In many different cancers, Sox2 has been associated with the metastasis and growth of tumors, mostly due to overexpression (PMID: 28388544). The increased levels of Sox2 RNA and protein in tumor tissue highlight it as a potential therapeutic target (PMID: 25114775).