Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HL-60 cells, HepG2 cells, K-562 cells, MDA-MB-231 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
27414-1-AP targets SOX4 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SOX4 fusion protein Ag26583 相同性解析による交差性が予測される生物種 |
| Full Name | SRY (sex determining region Y)-box 4 |
| Calculated molecular weight | 474 aa, 47 kDa |
| Observed molecular weight | 47 kDa |
| GenBank accession number | BC072668 |
| Gene Symbol | SOX4 |
| Gene ID (NCBI) | 6659 |
| RRID | AB_3085955 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q06945 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors that play key roles in the regulation of stemness, differentiation, progenitor cell development, and multiple developmental pathways. A single-exon gene encodes the SOX4 protein and contains a highly conserved high-mobility group (HMG) DNA binding domain related to the TCF/LEF family of transcription factors that play important roles in the Wnt pathway. SOX4 is expressed in stem cells, and modulates stem cell activation and likely also plays a role in stem cell maintenance, possibly through activation of expression of SOX2 (PMID: 23764001, 31445218). The calculated molecular weight of SOX4 is 47 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SOX4 antibody 27414-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) DNA Sensor ABCF1 Phase Separates With cccDNA to Inhibit Hepatitis B Virus Replication | ||
Int J Mol Sci Preclinical Repurposing of Sitagliptin as a Drug Candidate for Colorectal Cancer by Targeting CD24/CTNNB1/SOX4-Centered Signaling Hub | ||
Oral Dis USP14 promotes the cancer stem-like cell properties of OSCC via promoting SOX2 deubiquitination | ||