Validation Data Gallery
Tested Applications
| Positive WB detected in | U-251 cells, Jurkat cells, C6 cells |
| Positive IHC detected in | rat testis tissue, mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IHC | See 2 publications below |
| IF | See 5 publications below |
Product Information
14010-1-AP targets SOX6 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SOX6 fusion protein Ag5111 相同性解析による交差性が予測される生物種 |
| Full Name | SRY (sex determining region Y)-box 6 |
| Calculated molecular weight | 92 kDa |
| Observed molecular weight | 90 kDa |
| GenBank accession number | BC047064 |
| Gene Symbol | SOX6 |
| Gene ID (NCBI) | 55553 |
| RRID | AB_2196095 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35712 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The Sex-determining Region Y (SRY)-related high-mobilitygroup box 6 (SOX6) is a transcription factor that was discovered in 1990s while searching for Sry-related genes. SOX6 belongs to the subfamily D of SOX protein and can recognize the conserved sequence of "(A/T)(A/T)CAA(A/ T)G" within the transcriptional regulation region of the target gene with its HMG domain. Sox6 contains DNA binding domain and binds to the minor groove of DNA. The ability of SOX6 to interact with different proteins at different places and times, makes it an intricate protein with roles in multiple processes. SOX6 has been implicated in diabetic nephropathy, adipogenesis, cardiomyopathy, inflammatory bowel disease, and cancer. (PMID: 33230925)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SOX6 antibody 14010-1-AP | Download protocol |
| IHC protocol for SOX6 antibody 14010-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination. | ||
Int J Mol Med miRNA‑342 suppresses renal interstitial fibrosis in diabetic nephropathy by targeting SOX6. | ||
Nephrology (Carlton) CircHOMER1 aggravates oxidative stress, inflammation and ECM deposition in high glucose-induced human mesangial cells | ||
bioRxiv Eed controls craniofacial osteoblast differentiation and mesenchymal proliferation from the neural crest | ||
Matrix Biol SERPINA3 is a marker of cartilage differentiation and is essential for the expression of extracellular matrix genes during early chondrogenesis |