Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells, mouse heart tissue, PC-3 cells |
| Positive IHC detected in | human prostate cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
23925-1-AP targets SOX7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SOX7 fusion protein Ag10323 相同性解析による交差性が予測される生物種 |
| Full Name | SRY (sex determining region Y)-box 7 |
| Calculated molecular weight | 388 aa, 42 kDa |
| Observed molecular weight | 42-45 kDa |
| GenBank accession number | BC071947 |
| Gene Symbol | SOX7 |
| Gene ID (NCBI) | 83595 |
| RRID | AB_2879363 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BT81 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SOX7, also named as Transcription factor SOX-7, is a 388 amino acid protein, which is widely expressed in adult and fetal tissues and tends to be down-regulated in prostate adenocarcinomas and colorectal tumors due to promoter hypermethylation. SOX7 binds to and activates the CDH5 promoter, hence plays a role in the transcriptional regulation of genes expressed in the hemogenic endothelium and blocks further differentiation into blood precursors. SOX7 may be required for the survival of both hematopoietic and endothelial precursors during specification.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SOX7 antibody 23925-1-AP | Download protocol |
| IHC protocol for SOX7 antibody 23925-1-AP | Download protocol |
| IF protocol for SOX7 antibody 23925-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
FASEB J System analysis based on weighted gene co-expression analysis identifies SOX7 as a novel regulator of hepatic stellate cell activation and liver fibrosis | ||
Mol Genet Genomics Predisposition to atrioventricular septal defects may be caused by SOX7 variants that impair interaction with GATA4. | ||
Cell Death Dis The transcription factor Sox7 modulates endocardiac cushion formation contributed to atrioventricular septal defect through Wnt4/Bmp2 signaling. | ||
Int J Biol Macromol Integrated transcriptomics and lipidomics reveals protective effect in vascular endothelial barrier of a polysaccharide from Typhae Pollen |