Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue, COLO 320 cells, rat brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
20627-1-AP targets SOX8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SOX8 fusion protein Ag14556 相同性解析による交差性が予測される生物種 |
| Full Name | SRY (sex determining region Y)-box 8 |
| Calculated molecular weight | 446 aa, 47 kDa |
| Observed molecular weight | 47-55 kDa |
| GenBank accession number | BC031797 |
| Gene Symbol | SOX8 |
| Gene ID (NCBI) | 30812 |
| RRID | AB_10693538 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P57073 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SOX8 antibody 20627-1-AP | Download protocol |
| IHC protocol for SOX8 antibody 20627-1-AP | Download protocol |
| IF protocol for SOX8 antibody 20627-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Thymic epithelial cells co-opt lineage-defining transcription factors to eliminate autoreactive T cells. | ||
Carcinogenesis SOX8 acts as a prognostic factor and mediator to regulate the progression of triple-negative breast cancer. | ||
Redox Biol Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis
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