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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SOX9 Polyclonal antibody (55152-1-AP)

    WB analysis using 55152-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55152-1-AP (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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SOX9 Polyclonal antibody

SOX9 Polyclonal Antibody for WB, ELISA
Cat No. 55152-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Transcription factor SOX-9, Transcription factor SOX 9, SRA1, CMPD1, CMD1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, TT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55152-1-AP targets SOX9 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name SRY (sex determining region Y)-box 9
Calculated molecular weight 56 kDa
Observed molecular weight56 kDa, 68-75 kDa
GenBank accession numberNM_000346
Gene Symbol SOX9
Gene ID (NCBI) 6662
RRIDAB_3670240
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48436
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SOX9, also named as Transcription factor SOX-9, is a 509 amino acid protein. Sex-determining region Y (SRY)-box 9 protein (SOX9) is a member of the SOX family of transcription factors (TFs) which are developmental regulators that possess high mobility group (HMG) box DNA binding and transactivation domains. It participates in a variety of functions, such as lineage restriction and terminal diferentiation, through precise temporal and spatial expression patterns that difer between particular cell types and tissues. SOX9 gene has been implicated in different types of cancer as an oncogene; however, it also may behave as a tumor suppressor. SOX9 can be ubiquitinated or SUMOylated to higher molecular weight (PMID: 24155239; 16307912; 16554309; 32070068)

Protocols

Product Specific Protocols
WB protocol for SOX9 antibody 55152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols