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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SP100 Polyclonal antibody (11377-1-AP)

    WB analysis of Jurkat using 11377-1-AP (same clone as 11377-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11377-1-AP (SP100 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11377-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using SP100 Polyclonal antibody (11377-1-AP)

    IHC staining of human ovary tumor using 11377-1-AP (same clone as 11377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11377-1-AP (SP100 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11377-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using SP100 Polyclonal antibody (11377-1-AP)

    IHC staining of human ovary tumor using 11377-1-AP (same clone as 11377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11377-1-AP (SP100 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11377-1-PBS in a different storage buffer formulation.

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SP100 Polyclonal antibody, PBS Only

SP100 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11377-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11377-1-PBS targets SP100 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SP100 fusion protein Ag1939 相同性解析による交差性が予測される生物種
Full Name SP100 nuclear antigen
Calculated molecular weight 879 aa, 100 kDa
Observed molecular weight 70-100 kDa, 110-130 kDa
GenBank accession numberBC011562
Gene Symbol SP100
Gene ID (NCBI) 6672
RRIDAB_2194302
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23497
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains. Sp100 is an acidic protein that exerts a highly aberrant electrophoretic mobility in SDS-polyacrylamide gel electrophoresis.