Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31102-1-AP targets SP100 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SP100 fusion protein Ag35193 相同性解析による交差性が予測される生物種 |
| Full Name | SP100 nuclear antigen |
| Calculated molecular weight | 879 aa, 100 kDa |
| Observed molecular weight | 75-85 kDa |
| GenBank accession number | BC011562 |
| Gene Symbol | SP100 |
| Gene ID (NCBI) | 6672 |
| RRID | AB_3669856 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P23497 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SP100 antibody 31102-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |