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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SP100 Recombinant antibody, PBS Only

SP100 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241153F8

Cat no : 84012-5-PBS

Synonyms

Speckled 100 kDa, SP100 nuclear antigen, SP 100, Nuclear dot-associated Sp100 protein, Nuclear autoantigen Sp-100

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SP100 Recombinant antibody (84012-5-RR)

    WB analysis using 84012-5-RR (same clone as 84012-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84012-5-RR (SP100 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84012-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SP100 Recombinant antibody (84012-5-RR)

    IF Staining of A431 using 84012-5-RR (same clone as 84012-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SP100 antibody (84012-5-RR, Clone: 241153F8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84012-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SP100 Recombinant antibody (84012-5-RR)

    IF Staining of A431 using 84012-5-RR (same clone as 84012-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SP100 antibody (84012-5-RR, Clone: 241153F8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84012-5-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84012-5-PBS is SP100 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SP100 fusion protein Ag35193 相同性解析による交差性が予測される生物種
Full Name SP100 nuclear antigen
Calculated molecular weight 879 aa, 100 kDa
Observed molecular weight70-100 kDa, 110-140 kDa
GenBank accession numberBC011562
Gene symbol SP100
Gene ID (NCBI) 6672
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SP100 nuclear antigen, belongs to the nuclear bodies(NBs), has a role in the control of gene expression. It contains a HSR domain, which is important for the nuclear body targeting as well as for the dimerization, and one PxVxL motif, which if required for interaction with chromoshadow domains. Sp100 is an acidic protein that exerts a highly aberrant electrophoretic mobility in SDS-polyacrylamide gel electrophoresis.