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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SP140L Polyclonal antibody (19845-1-AP)

    WB analysis of HeLa using 19845-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19845-1-AP (SP140L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using SP140L Polyclonal antibody (19845-1-AP)

    IP experiment of HeLa using 19845-1-AP

    IP result of anti-SP140L (IP:19845-1-AP, 4ug; Detection:19845-1-AP 1:500) with HeLa cells lysate 3200ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using SP140L Polyclonal antibody (19845-1-AP)

    IP experiment of HeLa using 19845-1-AP

    IP result of anti-SP140L (IP:19845-1-AP, 4ug; Detection:19845-1-AP 1:500) with HeLa cells lysate 3200ug.

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SP140L Polyclonal antibody

SP140L Polyclonal Antibody for WB, IP, ELISA
Cat No. 19845-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

SP140L

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19845-1-AP targets SP140L in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SP140L fusion protein Ag13894 相同性解析による交差性が予測される生物種
Full Name SP140 nuclear body protein-like
Calculated molecular weight 438 aa, 50 kDa
Observed molecular weight 67-77 kDa
GenBank accession numberBC004921
Gene Symbol SP140L
Gene ID (NCBI) 93349
RRIDAB_2878613
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H930
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SP140L antibody 19845-1-APDownload protocol
IP protocol for SP140L antibody 19845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols