• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SP6 Polyclonal antibody

SP6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28561-1-AP

Synonyms

Krueppel-like factor 14, KLF14, EPIPROFIN, EPFN, AI1K

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SP6 Polyclonal antibody (28561-1-AP)

    WB analysis using 28561-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28561-1-AP (SP6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SP6 Polyclonal antibody (28561-1-AP)

    IHC staining of human stomach cancer using 28561-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28561-1-AP (SP6 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SP6 Polyclonal antibody (28561-1-AP)

    IF Staining of HepG2 using 28561-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SP6 antibody (28561-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

☆1/31（金）までお得に買える！ >> IHC・ELISA・IPキット最大30%OFFキャンペーン

Tested Applications

Positive WB detected inL02 cells, MCF-7 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28561-1-AP targets SP6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SP6 fusion protein Ag29372 相同性解析による交差性が予測される生物種
Full Name Sp6 transcription factor
Calculated molecular weight 376 aa, 40 kDa
Observed molecular weight40-44 kDa
GenBank accession numberBC103951
Gene symbol SP6
Gene ID (NCBI) 80320
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SP6 antibody 28561-1-APDownload protocol
IHC protocol for SP6 antibody 28561-1-APDownload protocol
IF protocol for SP6 antibody 28561-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols