SPACA3 Polyclonal antibody
SPACA3 Polyclonal Antibody for WB, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 21137-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, mouse testis tissue |
Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 21137-1-AP is SPACA3 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SPACA3 fusion protein Ag15589 相同性解析による交差性が予測される生物種 |
Full Name | sperm acrosome associated 3 |
Calculated molecular weight | 215 aa, 23 kDa |
Observed molecular weight | 28-32 kDa, 46-50 kDa |
GenBank accession number | BC029867 |
Gene symbol | SPACA3 |
Gene ID (NCBI) | 124912 |
RRID | AB_10913810 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SPACA3, also named as LYC3, LYZL3, SLLP1, SPRASA, CT54 and ALLP-17, belongs to the glycosyl hydrolase 22 family. It is a sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. SPACA3 has a Dimer form(PMID:15982649 ).
Protocols
Product Specific Protocols | |
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WB protocol for SPACA3 antibody 21137-1-AP | Download protocol |
IF protocol for SPACA3 antibody 21137-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |