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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPACA3 Polyclonal antibody

SPACA3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21137-1-AP

Synonyms

Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17, Lysozyme like protein 3, LYC3, CT54, Cancer/testis antigen 54

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SPACA3 Polyclonal antibody (21137-1-AP)

    WB analysis of HEK-293 using 21137-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21137-1-AP (SPACA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using SPACA3 Polyclonal antibody (21137-1-AP)

    WB analysis of mouse testis using 21137-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21137-1-AP (SPACA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SPACA3 Polyclonal antibody (21137-1-AP)

    WB analysis of HEK-293 using 21137-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21137-1-AP (SPACA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SPACA3 Polyclonal antibody (21137-1-AP)

    IF Staining of HEK-293 using 21137-1-AP

    Immunofluorescent analysis of HEK-293 cells using 21137-1-AP (SPACA3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21137-1-AP targets SPACA3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPACA3 fusion protein Ag15589 相同性解析による交差性が予測される生物種
Full Name sperm acrosome associated 3
Calculated molecular weight 215 aa, 23 kDa
Observed molecular weight 28-32 kDa, 46-50 kDa
GenBank accession numberBC029867
Gene symbol SPACA3
Gene ID (NCBI) 124912
RRIDAB_10913810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPACA3, also named as LYC3, LYZL3, SLLP1, SPRASA, CT54 and ALLP-17, belongs to the glycosyl hydrolase 22 family. It is a sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. SPACA3 has a Dimer form(PMID:15982649 ).

Protocols

Product Specific Protocols
WB protocol for SPACA3 antibody 21137-1-APDownload protocol
IF protocol for SPACA3 antibody 21137-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols