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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPAG11A Polyclonal antibody, PBS Only

SPAG11A Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16770-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human testis tissue using SPAG11A Polyclonal antibody (16770-1-AP)

    WB analysis of human testis using 16770-1-AP (same clone as 16770-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 16770-1-AP (SPAG11A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using SPAG11A Polyclonal antibody (16770-1-AP)

    WB analysis of human brain using 16770-1-AP (same clone as 16770-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 16770-1-AP (SPAG11A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SPAG11A Polyclonal antibody (16770-1-AP)

    IHC staining of human testis using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using SPAG11A Polyclonal antibody (16770-1-AP)

    IHC staining of human testis using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using SPAG11A Polyclonal antibody (16770-1-AP)

    IHC staining of human skin using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using SPAG11A Polyclonal antibody (16770-1-AP)

    IHC staining of human skin using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using SPAG11A Polyclonal antibody (16770-1-AP)

    IHC staining of human spleen using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using SPAG11A Polyclonal antibody (16770-1-AP)

    IHC staining of human spleen using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using SPAG11A Polyclonal antibody (16770-1-AP)

    IHC staining of human lung using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using SPAG11A Polyclonal antibody (16770-1-AP)

    IHC staining of human lung using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using SPAG11A Polyclonal antibody (16770-1-AP)

    IHC staining of human ovary using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using SPAG11A Polyclonal antibody (16770-1-AP)

    IHC staining of human ovary using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SPAG11A Polyclonal antibody (16770-1-AP)

    IHC staining of human kidney using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SPAG11A Polyclonal antibody (16770-1-AP)

    IHC staining of human kidney using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SPAG11A Polyclonal antibody (16770-1-AP)

    IHC staining of human placenta using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SPAG11A Polyclonal antibody (16770-1-AP)

    IHC staining of human placenta using 16770-1-AP (same clone as 16770-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 16770-1-AP (SPAG11A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16770-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16770-1-PBS targets SPAG11A in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPAG11A fusion protein Ag9846 相同性解析による交差性が予測される生物種
Full Name sperm associated antigen 11A
Calculated molecular weight 123 aa, 14 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC058833
Gene symbol SPAG11A
Gene ID (NCBI) 653423
RRIDAB_2286582
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Sperm binding proteins and their C-terminal peptides of the Sperm Associated Antigen 11 (SPAG11) family are found to play an important role in epididymal innate immunity in addition to their role in sperm maturation(PMID:16643671).SPAG11 peptides resulting due to cleavage at the furin-like proprotein convertase motif are reported in the epididymal fluid, ejaculate and on sperm and their antimicrobial activity demonstrated.SPAG11A belongs to this family and is also named as EP2,HE2. It has 4 isoforms produced by alternative splicing.This antibody is specific to SPAG11.