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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPAG5 Recombinant antibody (84809-4-RR)

    WB analysis using 84809-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84809-4-RR (SPAG5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84809-4-RR

    Affinity and Kinetic Characterization of 84809-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84809-4-RR against Human SPAG5 were performed. The affinity constant is below 1 pM.

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SPAG5 Recombinant antibody

SPAG5 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84809-4-RR
Clone No.242374H6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Mitotic spindle-associated protein p126, MAP126, hMAP126, Deepest, Astrin

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, A549 cells, PC-3 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84809-4-RR targets SPAG5 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SPAG5 fusion protein Ag6485 相同性解析による交差性が予測される生物種
Full Name sperm associated antigen 5
Calculated molecular weight 134 kDa
Observed molecular weight 135 kDa, 150 kDa
GenBank accession numberBC000322
Gene Symbol SPAG5
Gene ID (NCBI) 10615
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96R06
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPAG5, also named as MAP126, Astrin and Deepest, is necessary for normal spindle formation during mitosis. It is highly expressed in testis. SPAG5 has different localization in somatic versus male germ cells suggesting the possibility of different function. This antibody is specific to SPAG5.

Protocols

Product Specific Protocols
WB protocol for SPAG5 antibody 84809-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols