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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using SPAM1 Polyclonal antibody (11208-1-AP)

    WB analysis of PC-3 using 11208-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11208-1-AP (SPAM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SPAM1 Polyclonal antibody (11208-1-AP)

    WB analysis of MCF-7 using 11208-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11208-1-AP (SPAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using SPAM1 Polyclonal antibody (11208-1-AP)

    WB analysis of human testis using 11208-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 11208-1-AP (SPAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SPAM1 Polyclonal antibody

SPAM1 Polyclonal Antibody for WB, ELISA
Cat No. 11208-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

HYA1, Hyal PH20, HYAL1, HYAL3, HYAL5, Hyaluronoglucosaminidase PH 20, PH 20, PH20, SPAG15, SPAM1, Sperm adhesion molecule 1, Sperm surface protein PH 20

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells, human testis tissue, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11208-1-AP targets SPAM1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPAM1 fusion protein Ag1682 相同性解析による交差性が予測される生物種
Full Name SPAM1
Calculated molecular weight 58 kDa
Observed molecular weight 45-47 kDa
GenBank accession numberBC026163
Gene Symbol SPAM1
Gene ID (NCBI) 6677
RRIDAB_2194936
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP38567
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPAM1(sperm adhesion molecule 1), also named as Hyal PH20, belongs to the glycosyl hydrolase 56 family. SPAM1 is a GPI-anchored enzyme present on both the sperm plasma membrane and acrosomal membrane. It is involved in sperm-egg adhesion.

Protocols

Product Specific Protocols
WB protocol for SPAM1 antibody 11208-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
bovineWB

Int J Biol Macromol

TMT-based quantitative N-glycoproteomic analysis reveals glycoprotein protection can improve the quality of frozen bovine sperm.

Authors - Na Wang
View Article