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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPAM1 Polyclonal antibody

SPAM1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11208-1-AP

Synonyms

HYA1, Hyal PH20, HYAL1, HYAL3, HYAL5, Hyaluronoglucosaminidase PH 20, PH 20, PH20, SPAG15, SPAM1, Sperm adhesion molecule 1, Sperm surface protein PH 20

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using SPAM1 Polyclonal antibody (11208-1-AP)

    WB analysis of PC-3 using 11208-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11208-1-AP (SPAM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SPAM1 Polyclonal antibody (11208-1-AP)

    WB analysis of MCF-7 using 11208-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11208-1-AP (SPAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using SPAM1 Polyclonal antibody (11208-1-AP)

    WB analysis of human testis using 11208-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 11208-1-AP (SPAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inPC-3 cells, human testis tissue, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11208-1-AP targets SPAM1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPAM1 fusion protein Ag1682 相同性解析による交差性が予測される生物種
Full Name SPAM1
Calculated molecular weight 58 kDa
Observed molecular weight 45-47 kDa
GenBank accession numberBC026163
Gene symbol SPAM1
Gene ID (NCBI) 6677
RRIDAB_2194936
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPAM1(sperm adhesion molecule 1), also named as Hyal PH20, belongs to the glycosyl hydrolase 56 family. SPAM1 is a GPI-anchored enzyme present on both the sperm plasma membrane and acrosomal membrane. It is involved in sperm-egg adhesion.

Protocols

Product Specific Protocols
WB protocol for SPAM1 antibody 11208-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
bovineWB

Int J Biol Macromol

TMT-based quantitative N-glycoproteomic analysis reveals glycoprotein protection can improve the quality of frozen bovine sperm.

Authors - Na Wang
View Article