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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using SPANXE Polyclonal antibody (15870-1-AP)

    WB analysis of A375 using 15870-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 15870-1-AP (SPANXE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using SPANXE Polyclonal antibody (15870-1-AP)

    WB analysis of mouse skin using 15870-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 15870-1-AP (SPANXE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using SPANXE Polyclonal antibody (15870-1-AP)

    IF Staining of A375 using 15870-1-AP

    Immunofluorescent analysis of A375 cells using 15870-1-AP (SPANXE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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SPANXE Polyclonal antibody

SPANXE Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15870-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

SPANX C, SPANX A2, Nuclear-associated protein SPAN-Xc, CT11.3, Cancer/testis-associated protein CTp11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA375 cells, mouse skin tissue
Positive IF/ICC detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15870-1-AP targets SPANXE in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPANXE fusion protein Ag8572 相同性解析による交差性が予測される生物種
Full Name SPANX family, member E
Calculated molecular weight 97 aa, 11 kDa
Observed molecular weight 15-20 kDa
GenBank accession numberBC005382
Gene Symbol SPANXE
Gene ID (NCBI) 171489
RRIDAB_10951107
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TAD1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPANXE (Sperm protein associated with the nucleus on the X chromosome E), also known as SPANX A2 or SPANX C, is a member of the SPANX family. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene encodes a sperm protein that contains a consensus nuclear localization signal but, although a role in spermatogenesis is suggested, the specific function of this family member has not yet been determined.

Protocols

Product Specific Protocols
WB protocol for SPANXE antibody 15870-1-APDownload protocol
IF protocol for SPANXE antibody 15870-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols