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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPARC Recombinant antibody (84621-3-RR)

    WB analysis using 84621-3-RR (same clone as 84621-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84621-3-RR (SPARC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84621-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01430-2

    Sandwich ELISA standard curve of MP01430-2

    Sandwich ELISA standard curve of MP01430-2, Human SPARC Recombinant Matched Antibody Pair - PBS only. 84621-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1169. 84621-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-1250 pg/mL

  • Sample test of MP01430-2

    Sample test of MP01430-2

    Serum of four healthy human donors was measured. The human SPARC concentration of detected samples was determined to be 212,811.0 pg/mL with a range of 153,761.1 - 333,381.8 pg/mL.

  • Sample test of MP01430-2

    Sample test of MP01430-2

    MG-63 cells were cultured in DMEM supplemented with 10% fetal bonive serum. An aliquot of the cell culture supernate was removed, assayed for human SPARC, and measured 91,240.8 pg/mL.

  • Sample test of MP01430-2

    Sample test of MP01430-2

    Human milk of four healthy human donors was measured. The human SPARC concentration of detected samples was determined to be 1,084.0 pg/mL with a range of 425.0 - 1,594.2 pg/mL.

  • Cytometric bead array standard curve of MP01430-2

    Cytometric bead array standard curve of MP01430-2

    Cytometric bead array standard curve of MP01430-2, SPARC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84621-1-PBS. Detection antibody: 84621-3-PBS. Standard: Eg1169. Range: 0.313-10 ng/mL.

  • Affinity and Kinetic Characterization of 84621-3-RR

    Affinity and Kinetic Characterization of 84621-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84621-3-RR against Human SPARC were performed. The affinity constant is 1.55 nM.

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SPARC Recombinant antibody, PBS Only (Detector)

SPARC Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84621-3-PBS
Clone No.241952E11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

ON, BM-40, BM 40, Basement-membrane protein 40, Basement membrane protein 40

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84621-3-PBS targets SPARC as part of a matched antibody pair:

MP01430-2: 84621-1-PBS capture and 84621-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name secreted protein, acidic, cysteine-rich (osteonectin)
Calculated molecular weight35kDa
Observed molecular weight40-43 kDa
GenBank accession numberNM_003118.4
Gene Symbol SPARC
Gene ID (NCBI) 6678
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09486
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SPARC, also known as ON (Osteonectin) or BM-40 (Basement-membrane protein 40), is an extracellular glycoprotein with the calculated molecular mass of 35 kDa and the apparent molecular mass of 40-43 kDa and 50 kDa (PMID: 7495300, 12365801). SPARC belongs to a group of matricellular proteins defined as secreted components that do not contribute directly to the formation of structural elements but serve to modulate cell-matrix interactions and cellular functions (PMID: 7542656; 12231357). SPARC is expressed at high levels in bone tissue, is distributed widely in many other tissues and cell types, and is associated generally with tissues undergoing morphogenesis, remodeling and wound repair (PMID: 10567433). It elicits changes in cell shape, inhibits cell-cycle progression, and influences the synthesis of extracellular matrix (PMID: 12721366). Altered expression of SPARC has been reported in a variety of cancers, which include breast, ovarian, colorectal, and pancreatic cancer as well as melanoma and glioblastomas (PMID: 18849185).