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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPATA13 Polyclonal antibody

SPATA13 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21400-1-AP

Synonyms

Spermatogenesis-associated protein 13, spermatogenesis associated 13, Asef2

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using SPATA13 Polyclonal antibody (21400-1-AP)

    WB analysis of COLO 320 using 21400-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 21400-1-AP (SPATA13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using SPATA13 Polyclonal antibody (21400-1-AP)

    WB analysis of human placenta using 21400-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 21400-1-AP (SPATA13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SPATA13 Polyclonal antibody (21400-1-AP)

    IF Staining of HepG2 using 21400-1-AP

    Immunofluorescent analysis of HepG2 cells, using SPATA13 antibody 21400-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SPATA13 Polyclonal antibody (21400-1-AP)

    IF Staining of HEK-293 using 21400-1-AP

    Immunofluorescent analysis of 293 cells, using SPATA13 antibody 21400-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SPATA13 Polyclonal antibody (21400-1-AP)

    IF Staining of HeLa using 21400-1-AP

    Immunofluorescent analysis of Hela cells, using SPATA13 antibody 21400-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inCOLO 320 cells, human placenta tissue
Positive IF/ICC detected inHepG2 cells, HEK-293 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21400-1-AP targets SPATA13 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPATA13 fusion protein Ag16101 相同性解析による交差性が予測される生物種
Full Name spermatogenesis associated 13
Calculated molecular weight 652 aa, 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC109291
Gene symbol SPATA13
Gene ID (NCBI) 221178
RRIDAB_10859783
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SPATA13 antibody 21400-1-APDownload protocol
IF protocol for SPATA13 antibody 21400-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols