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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPATA2 Polyclonal antibody

SPATA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29321-1-AP

Synonyms

FLJ13167, KIAA0757, PD1, SPATA2, spermatogenesis associated 2, tamo

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPATA2 Polyclonal antibody (29321-1-AP)

    WB analysis using 29321-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29321-1-AP (SPATA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using SPATA2 Polyclonal antibody (29321-1-AP)

    IHC staining of mouse lung using 29321-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29321-1-AP (SPATA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using SPATA2 Polyclonal antibody (29321-1-AP)

    IHC staining of mouse lung using 29321-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29321-1-AP (SPATA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SPATA2 Polyclonal antibody (29321-1-AP)

    IF Staining of HeLa using 29321-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPATA2 antibody (29321-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29321-1-AP targets SPATA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPATA2 fusion protein Ag30773 相同性解析による交差性が予測される生物種
Full Name spermatogenesis associated 2
Calculated molecular weight 58 kDa
Observed molecular weight58-60 kDa
GenBank accession numberBC009481
Gene symbol SPATA2
Gene ID (NCBI) 9825
RRIDAB_2918282
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The protein SPATA2 (spermatogenesis-associated protein 2) was initially shown to be highly expressed in testicular Sertoli cells, and these early reports suggested SPATA2 to play a role in spermatogenesis. More recently, it was discussed to be involved in the predisposition for psoriasis. SPATA2 is required for TNF-induced cell death. SPATA2 has a potential role during pancreatic development and β-cell proliferation.(PMID: 20119533/PMID: 27458237)

Protocols

Product Specific Protocols
WB protocol for SPATA2 antibody 29321-1-APDownload protocol
IHC protocol for SPATA2 antibody 29321-1-APDownload protocol
IF protocol for SPATA2 antibody 29321-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols