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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPATS2L Polyclonal antibody

SPATS2L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29930-1-AP

Synonyms

DKFZp564A2416, DNAPTP6, LOC26010, SPATS2 like protein, SPATS2L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPATS2L Polyclonal antibody (29930-1-AP)

    WB analysis using 29930-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29930-1-AP (SPATS2L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SPATS2L Polyclonal antibody (29930-1-AP)

    WB analysis using 29930-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29930-1-AP (SPATS2L antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SPATS2L Polyclonal antibody (29930-1-AP)

    IHC staining of human placenta using 29930-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SPATS2L Polyclonal antibody (29930-1-AP)

    IHC staining of human placenta using 29930-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SPATS2L Polyclonal antibody (29930-1-AP)

    IHC staining of human lung cancer using 29930-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SPATS2L Polyclonal antibody (29930-1-AP)

    IHC staining of human lung cancer using 29930-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29930-1-AP (SPATS2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SPATS2L Polyclonal antibody (29930-1-AP)

    IF Staining of HepG2 using 29930-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SPATS2L antibody (29930-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SPATS2L Polyclonal antibody (29930-1-AP)

    IF Staining of HepG2 using 29930-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SPATS2L antibody (29930-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SPATS2L Polyclonal antibody (29930-1-AP)

    IF Staining of HepG2 using 29930-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SPATS2L antibody (29930-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inA549 cells, HepG2 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman lung cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29930-1-AP targets SPATS2L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPATS2L fusion protein Ag31157 相同性解析による交差性が予測される生物種
Full Name viral DNA polymerase-transactivated protein 6
Calculated molecular weight 488 aa, 54 kDa
Observed molecular weight54-65 kDa
GenBank accession numberBC018736
Gene symbol SPATS2L
Gene ID (NCBI) 26010
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPATS2L encodes spermatogenesis associated and serine-rich 2-like protein, also named DNAPTP6 (DNA polymerase transactivated protein 6) in human (PMID:11230166). SPATS2L acts as an important regulator of β2-adrenergic receptor down-regulation (PubMed: 25562107). MiR-1269a down-regulates the expression of SPATS2L and LRP6, thereby inhibiting the proliferation of liver cancer cells (PMID: 28081866, PMID:35252180). SPATS2L has several isoforms by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for SPATS2L antibody 29930-1-APDownload protocol
IHC protocol for SPATS2L antibody 29930-1-APDownload protocol
IF protocol for SPATS2L antibody 29930-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols