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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SPEG Polyclonal antibody (18716-1-AP)

    IHC staining of mouse skeletal muscle using 18716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using SPEG Polyclonal antibody (18716-1-AP)

    IHC staining of mouse skeletal muscle using 18716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using SPEG Polyclonal antibody (18716-1-AP)

    IHC staining of mouse heart using 18716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using SPEG Polyclonal antibody (18716-1-AP)

    IHC staining of mouse heart using 18716-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18716-1-AP (SPEG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SPEG Polyclonal antibody

SPEG Polyclonal Antibody for IHC, ELISA
Cat No. 18716-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

APEG 1, APEG1, BPEG, KIAA1297, SPEG, SPEG complex locus, SPEGalpha, SPEGbeta

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse skeletal muscle tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18716-1-AP targets SPEG in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name SPEG complex locus
Calculated molecular weight 354 kDa
GenBank accession numberNM_005876
Gene Symbol SPEG
Gene ID (NCBI) 10290
RRIDAB_3085566
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15772
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPEG, also named as BPEG, APEG1 and KIAA1297, is highly similar to the corresponding rat and mouse proteins. Expression of SPEG is thought to serve as a marker for differentiated vascular smooth muscle cells which may have a role in regulating growth and differentiation of this cell type. SPEG is expressed in vascular smooth muscle cells and is down-regulated by vascular injury. Multiple transcripts are produced from the SPEG complex locus by alternative splicing and alternative promoter usage. The antibody recognizes the C-term of SPEG.

Protocols

Product Specific Protocols
IHC protocol for SPEG antibody 18716-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols