Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, RAW264.7, mouse liver tissue, rat liver tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 5 publications below |
IF | See 1 publications below |
Product Information
55100-1-AP targets SPI1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | spleen focus forming virus (SFFV) proviral integration oncogene spi1 |
Calculated molecular weight | 31 kDa |
Observed molecular weight | 31-42 kDa |
GenBank accession number | NM_003120 |
Gene Symbol | SPI1 |
Gene ID (NCBI) | 6688 |
ENSEMBL Gene ID | ENSG00000066336 |
RRID | AB_10858486 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P17947 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SPI1 binds to the PU-box which is a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. SPI1 is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. It also binds RNA and may modulate pre-mRNA splicing. The antibody is specific to SPI1. SPI1 can be detected 37-42 kDa (PMID: 28362429, 31586032, 28586009).
Protocols
Product Specific Protocols | |
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WB protocol for SPI1 antibody 55100-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mucosal Immunol IL-33/ST2/IL-9/IL-9R signaling disrupts ocular surface barrier in allergic inflammation. | ||
Sci Rep Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine. | ||
J Leukoc Biol miR-199a-5p inhibits monocyte/macrophage differentiation by targeting the activin A type 1B receptor gene and finally reducing C/EBPα expression.
| ||
Exp Cell Res An innovative immunotherapeutic strategy for rheumatoid arthritis: Selectively suppressing angiogenesis and osteoclast differentiation by fully human antibody targeting thymocyte antigen-1 | ||
Cell Rep A CLIC1 network coordinates matrix stiffness and the Warburg effect to promote tumor growth in pancreatic cancer |