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  • KD/KO Validated

SPI1 Polyclonal antibody

SPI1 Polyclonal Antibody for WB, ELISA
Cat No. 55100-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

31 kDa transforming protein, OF, PU.1, SFPI1, SPI 1, SPI A, SPI1, Transcription factor PU.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHepG2 cells, RAW264.7, mouse liver tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55100-1-AP targets SPI1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name spleen focus forming virus (SFFV) proviral integration oncogene spi1
Calculated molecular weight 31 kDa
Observed molecular weight 31-42 kDa
GenBank accession numberNM_003120
Gene Symbol SPI1
Gene ID (NCBI) 6688
ENSEMBL Gene IDENSG00000066336
RRIDAB_10858486
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17947
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPI1 binds to the PU-box which is a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. SPI1 is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. It also binds RNA and may modulate pre-mRNA splicing. The antibody is specific to SPI1. SPI1 can be detected 37-42 kDa (PMID: 28362429, 31586032, 28586009).

Protocols

Product Specific Protocols
WB protocol for SPI1 antibody 55100-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Mucosal Immunol

IL-33/ST2/IL-9/IL-9R signaling disrupts ocular surface barrier in allergic inflammation.

Authors - Jiaoyue Hu
mouseWB

Sci Rep

Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine.

Authors - Yanhong Shi
humanWB

J Leukoc Biol

miR-199a-5p inhibits monocyte/macrophage differentiation by targeting the activin A type 1B receptor gene and finally reducing C/EBPα expression.

Authors - Hai-Shuang Lin
  • KD Validated
humanWB

Med Oncol

PU.1 negatively regulates tumorigenesis in non-small-cell lung cancer

Authors - Xiaoxiao Liu
humanWB

Exp Cell Res

An innovative immunotherapeutic strategy for rheumatoid arthritis: Selectively suppressing angiogenesis and osteoclast differentiation by fully human antibody targeting thymocyte antigen-1

Authors - Xuanxuan Hu
humanWB

Cell Rep

A CLIC1 network coordinates matrix stiffness and the Warburg effect to promote tumor growth in pancreatic cancer

Authors - Jia-Hao Zheng
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