Validation Data Gallery
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Tested Applications
| Positive WB detected in | L02 cells |
| Positive IP detected in | L02 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
15768-1-AP targets SPIB in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SPIB fusion protein Ag8404 相同性解析による交差性が予測される生物種 |
| Full Name | Spi-B transcription factor (Spi-1/PU.1 related) |
| Calculated molecular weight | 262 aa, 29 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC007921 |
| Gene Symbol | SPIB |
| Gene ID (NCBI) | 6689 |
| RRID | AB_2878182 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01892 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SPIB antibody 15768-1-AP | Download protocol |
| IHC protocol for SPIB antibody 15768-1-AP | Download protocol |
| IP protocol for SPIB antibody 15768-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Signal SPIB acts as a tumor suppressor by activating the NFkB and JNK signaling pathways through MAP4K1 in colorectal cancer cells. | ||
J Exp Clin Cancer Res tsRNA-GlyGCC promotes colorectal cancer progression and 5-FU resistance by regulating SPIB |