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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPIN1 Monoclonal antibody

SPIN1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G8C5

Cat no : 68615-1-Ig

Synonyms

OCR, Ovarian cancer related protein, SPIN, SPIN1, spindlin 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPIN1 Monoclonal antibody (68615-1-Ig)

    WB analysis using 68615-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68615-1-Ig (SPIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SPIN1 Monoclonal antibody (68615-1-Ig)

    WB analysis using 68615-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68615-1-Ig (SPIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCCIT cells using SPIN1 Monoclonal antibody (68615-1-Ig)

    WB analysis of NCCIT using 68615-1-Ig

    NCCIT cells were subjected to SDS PAGE followed by western blot with 68615-1-Ig (SPIN1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inLNCaP cells, NCCIT cells, rabbit brain tissue, SK-BR-3 cells, HeLa cells, Jurkat cells, K-562 cells, rat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68615-1-Ig targets SPIN1 in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Reactivity Human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SPIN1 fusion protein Ag33686 相同性解析による交差性が予測される生物種
Full Name spindlin 1
Calculated molecular weight 262 aa, 30 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC013571
Gene symbol SPIN1
Gene ID (NCBI) 10927
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPIN1 (Spindlin 1) is a histone methylation reader, which recognizes trimethylated histone H3 lysine 4 through the protein-protein interaction. SPIN1 was initially found in ovarian cancer via searching ovarian cancer-related genes . It is overexpressed in ovarian cancer tissue, which exhibits a high homology to mouse Spin (Spindlin) gene. Signaling pathways responsible for SPIN1 functions include PI3K/Akt, Wnt and RET that are highly relevant to tumorigenesis.(PMID: 34492335, PMID: 31790762)

Protocols

Product Specific Protocols
WB protocol for SPIN1 antibody 68615-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols