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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPIN2B Polyclonal antibody

SPIN2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10107-2-AP

Synonyms

SPIN-2B, SPIN2_duplicate, SPIN-2, SPIN2, SPIN 2B

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SPIN2B Polyclonal antibody (10107-2-AP)

    WB analysis of mouse brain using 10107-2-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10107-2-AP (SPIN2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SPIN2B Polyclonal antibody (10107-2-AP)

    IHC staining of human breast cancer using 10107-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10107-2-AP (SPIN2B antibody) at dilution of 1:50 (under 25x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SPIN2B Polyclonal antibody (10107-2-AP)

    IHC staining of human breast cancer using 10107-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10107-2-AP (SPIN2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SPIN2B Polyclonal antibody (10107-2-AP)

    IHC staining of human breast cancer using 10107-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10107-2-AP (SPIN2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using SPIN2B Polyclonal antibody (10107-2-AP)

    IHC staining of human colon using 10107-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10107-2-AP (SPIN2B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SPIN2B Polyclonal antibody (10107-2-AP)

    IF Staining of HeLa using 10107-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SPIN2B antibody (10107-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SPIN2B Polyclonal antibody (10107-2-AP)

    IF Staining of HEK-293 using 10107-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SPIN2B antibody (10107-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10107-2-AP targets SPIN2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPIN2B fusion protein Ag0157 相同性解析による交差性が予測される生物種
Full Name spindlin family, member 2B
Calculated molecular weight 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC000044
Gene symbol SPIN2B
Gene ID (NCBI) 474343
RRIDAB_2196120
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SPIN2B antibody 10107-2-APDownload protocol
IHC protocol for SPIN2B antibody 10107-2-APDownload protocol
IF protocol for SPIN2B antibody 10107-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols