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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using HAI-1 Polyclonal antibody (15036-1-AP)

    WB analysis of human placenta using 15036-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 15036-1-AP (HAI-1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HAI-1 Polyclonal antibody (15036-1-AP)

    IHC staining of human prostate cancer using 15036-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15036-1-AP (HAI-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HAI-1 Polyclonal antibody (15036-1-AP)

    IHC staining of human prostate cancer using 15036-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15036-1-AP (HAI-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using HAI-1 Polyclonal antibody (15036-1-AP)

    IF Staining of NIH/3T3 using 15036-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using HAI-1 antibody (15036-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

HAI-1 Polyclonal antibody

HAI-1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15036-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SPINT1, HAI 1, HAI, MANSC2, Kunitz-type protease inhibitor 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15036-1-AP targets HAI-1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HAI-1 fusion protein Ag6665 相同性解析による交差性が予測される生物種
Full Name serine peptidase inhibitor, Kunitz type 1
Calculated molecular weight 58 kDa
Observed molecular weight 58-70 kDa
GenBank accession numberBC004140
Gene Symbol HAI-1
Gene ID (NCBI) 6692
RRIDAB_2196284
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43278
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HAI-1 antibody 15036-1-APDownload protocol
IHC protocol for HAI-1 antibody 15036-1-APDownload protocol
IF protocol for HAI-1 antibody 15036-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols