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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using SPO11 Polyclonal antibody (15509-1-AP)

    WB analysis of mouse testis using 15509-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15509-1-AP (SPO11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using SPO11 Polyclonal antibody (15509-1-AP)

    WB analysis of human testis using 15509-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 15509-1-AP (SPO11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

SPO11 Polyclonal antibody

SPO11 Polyclonal Antibody for WB, ELISA
Cat No. 15509-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Cancer/testis antigen 35, CT35, SPO11

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, human testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15509-1-AP targets SPO11 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPO11 fusion protein Ag4340 相同性解析による交差性が予測される生物種
Full Name SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae)
Calculated molecular weight 45 kDa
Observed molecular weight 55-70 kDa
GenBank accession numberBC033591
Gene Symbol SPO11
Gene ID (NCBI) 23626
RRIDAB_10639508
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5K1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Spo11 is a protein used in a complex along with Mre11 and Rad50 during meiotic recombination. It is also involved in the creation of double stranded breaks in the DNA in the early stages of this process. Several transcript variants encoding different isoforms have been found for this gene.

Protocols

Product Specific Protocols
WB protocol for SPO11 antibody 15509-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols