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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPOCK1 Polyclonal antibody (28203-1-AP)

    WB analysis using 28203-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28203-1-AP (SPOCK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SPOCK1 Polyclonal antibody (28203-1-AP)

    IHC staining of human prostate cancer using 28203-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28203-1-AP (SPOCK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SPOCK1 Polyclonal antibody (28203-1-AP)

    IHC staining of human prostate cancer using 28203-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28203-1-AP (SPOCK1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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SPOCK1 Polyclonal antibody

SPOCK1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28203-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Synonyms

FLJ37170, Protein SPOCK, SPOCK, SPOCK1, TESTICAN, Testican 1, TIC1, TICN1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, PC-3 cells, DU 145 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28203-1-AP targets SPOCK1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPOCK1 fusion protein Ag28213 相同性解析による交差性が予測される生物種
Full Name sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1
Calculated molecular weight 439 aa, 49 kDa
Observed molecular weight49 kDa
GenBank accession numberBC030691
Gene Symbol SPOCK1
Gene ID (NCBI) 6695
RRIDAB_3086036
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08629
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SPOCK1 antibody 28203-1-APDownload protocol
IHC protocol for SPOCK1 antibody 28203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Heliyon

Extrachromosomal circular DNA-related SPOCK1 contributes to development and enzalutamide resistance of prostate cancer by regulating epithelial mesenchymal transition

Authors - Yicong Yao
View Article