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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SPOCK1 Recombinant antibody (84977-1-RR)

    WB analysis using 84977-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84977-1-RR (SPOCK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.ce

  • Affinity and Kinetic Characterization of 84977-1-RR

    Affinity and Kinetic Characterization of 84977-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84977-1-RR against Human SPOCK1 were performed. The affinity constant is 3.51 nM.

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SPOCK1 Recombinant antibody

SPOCK1 Recombinant Antibody for WB, ELISA
Cat No. 84977-1-RR
Clone No.242196E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Testican-1, Testican 1, TESTICAN, SPOCK, Protein SPOCK

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDU 145 cells, PC-3 eclls, HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84977-1-RR targets SPOCK1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SPOCK1 fusion protein Ag28204 相同性解析による交差性が予測される生物種
Full Name sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1
Calculated molecular weight 439 aa, 49 kDa
Observed molecular weight49 kDa
GenBank accession numberBC030691
Gene Symbol SPOCK1
Gene ID (NCBI) 6695
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ08629
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPOCK1, also known as TICN1. It is predicted to be located in the extracellular matrix. The proein may play a role in cell-cell and cell-matrix interactions and may contribute to various neuronal mechanisms in the central nervous system. The molecular weight of SPOCK1 is 49 kDa.

Protocols

Product Specific Protocols
WB protocol for SPOCK1 antibody 84977-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols