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Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using SPON2 Monoclonal antibody (66859-1-Ig)

    WB analysis of LNCaP using 66859-1-Ig (same clone as 66859-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66859-1-Ig (SPON2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66859-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SPON2 Monoclonal antibody (66859-1-Ig)

    IHC staining of human prostate cancer using 66859-1-Ig (same clone as 66859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66859-1-Ig (SPON2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66859-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SPON2 Monoclonal antibody (66859-1-Ig)

    IHC staining of human prostate cancer using 66859-1-Ig (same clone as 66859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66859-1-Ig (SPON2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66859-1-PBS in a different storage buffer formulation.

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SPON2 Monoclonal antibody, PBS Only

SPON2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66859-1-PBS
Clone No.1H4B11

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Synonyms

DIL 1, DIL1, M spondin, Mindin, SPON2, Spondin 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66859-1-PBS targets SPON2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SPON2 fusion protein Ag14337 相同性解析による交差性が予測される生物種
Full Name spondin 2, extracellular matrix protein
Calculated molecular weight 331 aa, 36 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC002707
Gene Symbol SPON2
Gene ID (NCBI) 10417
RRIDAB_2882198
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BUD6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SPON2 (Spondin 2), also known as Mindin, is a member of the F-spondin family of secreted extracellular matrix proteins. This protein has been implicated in axon guidance, immune response and adhesion. SPON2 is essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (PMID: 14691481). It also functions as an integrin ligand for inflammatory cell recruitment and T-cell priming (PMID: 19153605). Besides, SPON2 has been reported as a prognostic biomarker for ovarian and prostate cancer (PMID: 17490732; 22615945; 23665271).