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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using SPP Polyclonal antibody (20416-1-AP)

    WB analysis of HEK-293 using 20416-1-AP (same clone as 20416-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20416-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using SPP Polyclonal antibody (20416-1-AP)

    WB analysis of mouse kidney using 20416-1-AP (same clone as 20416-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20416-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using SPP Polyclonal antibody (20416-1-AP)

    WB analysis of U2OS using 20416-1-AP (same clone as 20416-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20416-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using SPP Polyclonal antibody (20416-1-AP)

    WB analysis of mouse liver using 20416-1-AP (same clone as 20416-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20416-1-AP (SPP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20416-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using SPP Polyclonal antibody (20416-1-AP)

    IHC staining of human hepatocirrhosis using 20416-1-AP (same clone as 20416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20416-1-AP (SPP antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20416-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using SPP Polyclonal antibody (20416-1-AP)

    IHC staining of human hepatocirrhosis using 20416-1-AP (same clone as 20416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20416-1-AP (SPP antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20416-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SPP Polyclonal antibody, PBS Only

SPP Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20416-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20416-1-PBS targets SPP in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPP fusion protein Ag14158 相同性解析による交差性が予測される生物種
Full Name histocompatibility (minor) 13
Calculated molecular weight 377 aa, 41 kDa
Observed molecular weight 45 kDa, 100 kDa
GenBank accession numberBC008938
Gene Symbol HM13/SPP
Gene ID (NCBI) 81502
RRIDAB_10665421
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TCT9
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.