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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPPL2A Polyclonal antibody

SPPL2A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13255-1-AP

Synonyms

IMP 3, IMP3, Intramembrane protease 3, Presenilin like protein 2, PSL2, SPP like 2A, SPPL2A

Validation Data Gallery

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  • Western Blot (WB) analysis of human colon tissue using SPPL2A Polyclonal antibody (13255-1-AP)

    WB analysis of human colon using 13255-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SPPL2A Polyclonal antibody (13255-1-AP)

    WB analysis of Jurkat using 13255-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using SPPL2A Polyclonal antibody (13255-1-AP)

    WB analysis of human colon using 13255-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using SPPL2A Polyclonal antibody (13255-1-AP)

    WB analysis of mouse colon using 13255-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SPPL2A Polyclonal antibody (13255-1-AP)

    WB analysis of Jurkat using 13255-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using SPPL2A Polyclonal antibody (13255-1-AP)

    IHC staining of human testis using 13255-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SPPL2A Polyclonal antibody (13255-1-AP)

    IHC staining of human testis using 13255-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SPPL2A Polyclonal antibody (13255-1-AP)

    IHC staining of human kidney using 13255-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SPPL2A Polyclonal antibody (13255-1-AP)

    IHC staining of human kidney using 13255-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SPPL2A Polyclonal antibody (13255-1-AP)

    IHC staining of human testis using 13255-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SPPL2A Polyclonal antibody (13255-1-AP)

    IHC staining of human testis using 13255-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inhuman colon tissue, Jurkat cells, mouse colon tissue
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13255-1-AP targets SPPL2A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPPL2A fusion protein Ag3935 相同性解析による交差性が予測される生物種
Full Name signal peptide peptidase-like 2A
Calculated molecular weight 520 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC025740
Gene symbol SPPL2A
Gene ID (NCBI) 84888
RRIDAB_2195000
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPPL2A(Signal peptide peptidase-like 2A) is also named as IMP3(Intramembrane protease 3), PSL2(Presenilin-like protein 2) and belongs to the peptidase A22B family. It is an aspartyl intramembrane protease, has been implicated in the proteolysis of TNF-alpha, Fas Ligand and Bri2 and endogenous signal-peptide-peptidase-like2A (SPPL2a) is localised in lysosomes/late endosomes(PMID:21896273). This protein has a signal peptide with 25 amino acid and seven glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for SPPL2A antibody 13255-1-APDownload protocol
IHC protocol for SPPL2A antibody 13255-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols