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- KD/KO Validated
SPRR3 Polyclonal antibody
SPRR3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 11742-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, human liver tissue |
Positive IP detected in | COLO 320 cells |
Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 8 publications below |
IHC | See 3 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
Product Information
11742-1-AP targets SPRR3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SPRR3 fusion protein Ag2337 相同性解析による交差性が予測される生物種 |
Full Name | small proline-rich protein 3 |
Calculated molecular weight | 19 kDa |
Observed molecular weight | 22-25 kDa |
GenBank accession number | BC017802 |
Gene symbol | SPRR3 |
Gene ID (NCBI) | 6707 |
RRID | AB_2195272 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SPRR3, also named as Esophagin, Cornifin beta and SPRC, belongs to the cornifin (SPRR) family. It is cross-linked envelope protein of keratinocytes. Increased expression of SPRR3 appears to be involved in colorectal tumorigenesis. SPRR3 is a marker for terminal squamous cell differentiation.a potential novel therapeutic target for less advanced stages of Breast Cancer.
Protocols
Product Specific Protocols | |
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WB protocol for SPRR3 antibody 11742-1-AP | Download protocol |
IHC protocol for SPRR3 antibody 11742-1-AP | Download protocol |
IF protocol for SPRR3 antibody 11742-1-AP | Download protocol |
IP protocol for SPRR3 antibody 11742-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
FASEB J Small proline-rich repeat 3 is a novel coordinator of PDGFRβ and integrin β1 crosstalk to augment proliferation and matrix synthesis by cardiac fibroblasts.
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Breast Cancer Res Treat Expression of SPRR3 is associated with tumor cell proliferation in less advanced stages of breast cancer. | ||
Arch Biochem Biophys Investigation of mouse amniotic fluid for stimulating ability of keratinocyte differentiation depending on the fetal stage. | ||
Onco Targets Ther Dysregulation of SPRR3/miR-876-3p Axis Contributes to Tumorigenesis in Non-Small-Cell Lung Cancer. | ||